日本平腹小蜂滞育幼虫的重要生化物质变化  

Physiochemical changes in diapause larvae of Anastatus japonicus Ashmead

作　　者：刘子欣 夏玥 吴其峰 赵灿 李敦松[1] LIU Zixin;XIA Yue;WU Qifeng;ZHAO Can;LI Dunsong(Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Plant Protection,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广州510640 [2]华南农业大学植物保护学院,广州510642

出　　处：《植物保护》2021年第6期158-165,共8页Plant Protection

基　　金：国家自然科学基金青年科学基金(32001968);广州市科技计划项目-基础与应用基础研究项目(博士青年科技人员类)(202102020211);广东省农业科学院院长基金(201930);国家现代农业产业技术体系(CARS-32-13)。

摘　　要：日本平腹小蜂是多种果树蝽类害虫的优势寄生蜂,能以老熟幼虫滞育越冬。本研究分别测定了日本平腹小蜂非滞育老熟幼虫、滞育25、35、45、55 d老熟幼虫和滞育解除老熟幼虫体内的总蛋白、脂类、糖类及醇类等生化物质的含量,以及海藻糖酶和抗氧化酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)等关键酶的活性变化,比较了日本平腹小蜂滞育、非滞育和滞育解除老熟幼虫体内重要生化物质的差异。结果表明:滞育老熟幼虫显著积累脂类、甘油三酯、糖原等能源物质以及山梨醇等低温保护物质,且滞育个体的抗氧化能力显著增强,这些变化有助于提高其抗逆性,满足滞育维持期及滞育解除后恢复发育的能量需求,保证滞育个体的发育和存活。结果为解析日本平腹小蜂的滞育机制奠定了基础。Anastatus japonicus Ashmead is the dominant parasitic wasp of various fruit tree stink bugs.It can overwinter through diapause of mature larvae.In this study,we measured the content of biochemical substances such as total protein,lipids,saccharides and alcohols,as well as enzyme activities of trehalase and antioxidant enzymes(SOD,POD,CAT)in the non-diapause mature larvae,diapausing mature larvae(25,35,45,55 d)and diapause-terminated mature larvae of A.japonicus.The results showed that energy substances resistant to low temperature,like lipids,triglycerides,glycogen and sorbitol were significantly accumulated in the diapause mature larvae.In addition,the antioxidant capability of diapause individuals was significantly enhanced.These changes may improve the resistance of A.japonicus to adverse environment,meet its energy requirements for the diapause maintenance and post-diapause development,and increase the survival ratio of diapause individuals.The results of this study provide information for understanding the mechanism of diapause of A.japonicus.

关 键 词：日本平腹小蜂 滞育 蛋白质 脂类 抗氧化酶 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]