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作 者:赵艳琴[1] 石凯[1] 张丽娟[1] 张冬梅[1] 于秀英[1] ZHAO Yanqin;SHI Kai;ZHANG Lijuan;ZHANG Dongmei;YU Xiuying(College of Agriculture,Inner Mongolia University for the Nationalities,Tongliao 028000,China)
出 处:《植物保护》2021年第6期196-200,共5页Plant Protection
基 金:内蒙古自然科学基金(2017MS0322);国家重点研发计划(2017YFD021000);内蒙古民族大学博士科研启动基金(BS396)。
摘 要:为明确高粱链格孢叶斑病菌Alternaria alternata的生物学特性,本文采用生长速率法研究了不同营养及环境条件对该病菌生长的影响。试验结果表明:不同培养基上菌丝生长有明显差异,其中马铃薯蔗糖琼脂培养基(PSA)最适合菌丝生长和孢子产生;不同碳、氮源条件下菌丝的生长有显著差异,其中最适宜菌丝生长碳源为葡萄糖,氮源是蛋白胨;该病原菌最适宜营养生长的温度为28℃,pH为7;菌丝的致死温度为47℃;光照时间对高粱叶斑病菌丝的营养生长没有影响。To clarify the biological characteristics of the pathogen causing Alternaria leaf spot in sorghum,the growth rate method was used to study the effects of different nutritional and environmental conditions on the growth of the pathogen.The results showed that the mycelial growth on different culture media was obviously different.PSA(potato sucrose agar)medium was the most suitable for hyphal growth and sporulation.Different carbon and nitrogen sources had significant effect on the growth of the pathogen,among which the most suitable carbon source was glucose,and the most suitable nitrogen source was peptone.The optimum temperature for mycelial growth was 28℃;the optimum pH was seven,and the lethal temperature waS47℃.Illumination time had no effect on the mycelial growth.
分 类 号:S435.14[农业科学—农业昆虫与害虫防治]
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