老年大鼠慢性阻塞性肺疾病模型建立后认知功能的变化  

Changes of Cognitive Function after Establishment of Chronic Obstructive Pulmonary Disease Model in Elderly Rats

作　　者：万朝晖[1] 刘冰[1] 景珩[1] 吴苏宁[1] WAN Zhaohui;LIU Bing;JING Heng;WU Suning(Jining First People’s Hospital,Jining 272100,China;不详)

机构地区：[1]山东省济宁市第一人民医院,山东济宁272100

出　　处：《中国医学创新》2021年第33期5-8,共4页Medical Innovation of China

基　　金：山东省医药卫生科技发展计划项目(2014WS0033)。

摘　　要：目的:探讨老年大鼠慢性阻塞性肺疾病模型建立后认知功能的变化。方法:选择60只老年雄性SD大鼠,按照随机数字表法分为COPD组(30只)和对照组(30只)。COPD组大鼠采用气道内滴注脂多糖和香烟烟熏法建立慢性阻塞性肺疾病模型。模型建立后,采用HE染色法观察两组的组织形态学变化。在慢性阻塞性肺疾病模型建立后的第1、7、14、30天,采用Morris水迷宫系统检测两组的平均逃避潜伏期。采用t检验和重复测量方差分析比较两组平均逃避潜伏期的差异和变化。结果:实验期间,COPD组3只大鼠死亡,对照组2只大鼠死亡,其余大鼠存活良好。HE染色显示两组间有显著性差异。干预前,两组平均逃避潜伏期比较,差异无统计学意义(P>0.05);慢性阻塞性肺疾病模型建立后第1、7、14、30天,COPD组老年大鼠的平均逃避潜伏期均明显长于对照组(P<0.05),差异逐渐增大。结论:慢性阻塞性肺疾病模型建立后,老年大鼠的平均逃避潜伏期增加,认知功能下降,随着时间的推移,老年大鼠的认知功能越来越差,应早期进行临床干预。Objective:To explore the changes of cognitive function of aged rats after the establishment of chronic obstructive pulmonary disease model.Method:A total of 60 aged male SD rats were selected and divided into the COPD group(30 rats)and the control group(30 rats)according to the method of random number table.The COPD group rats were established COPD model by intratracheal instillation of lipopolysaccharide and fumigation.After the establishment of the model,HE staining was used to observe the histopathological changes of the two groups.On the first day and the 7th,14th and 30th day after the establishment of the COPD model,Morris water maze system was used to detect the average escape latency of the two groups,t-test and repeated measurement analysis of variance were used to compare the differences and changes of the average escape latency of the two groups.Result:During the experiment,3 rats in the COPD group died,2 rats in the control group died,and the other rats survived well.HE staining showed that there was significant difference between the two groups.Before intervention,there was no significant difference in the average escape latency between the two groups(P>0.05);on the first day,the 7th,14th and 30th day after the establishment of COPD model,the average escape latency time of the old rats in the COPD group were significantly longer than those of the control group(P<0.05),the difference gradually increased.Conclusion:After the establishment of COPD model,the average escape latency of elderly rats increases and the cognitive function decreases,with the passage of time,the cognitive function of elderly rats becomes worse and worse,so clinical intervention should be carried out early.

关 键 词：慢性阻塞性肺疾病 老年 认知功能 

分 类 号：R563.9[医药卫生—呼吸系统] R-332[医药卫生—内科学]