高效液相法测定化妆品中对乙酰氨基酚  

Determination of acetaminophen in cosmetics by high performance liquid chromatography

作　　者：张江维[1] 张弦[1] 肖树雄[1] Zhang Jiangwei;Zhang Xian;Xiao Shuxiong(Guangdong Institute for Drug Control,Guangzhou 510663,China)

机构地区：[1]广东省药品检验所,广州510663

出　　处：《化学分析计量》2021年第12期13-17,共5页Chemical Analysis And Meterage

摘　　要：建立高效液相色谱法测定化妆品中对乙酰氨基酚的方法,并用液相色谱-质谱联用仪对疑似结果进行确证分析。样品经甲醇超声提取,使用C_(18)色谱柱(250 mm×4.6 mm,5μm)分离,以甲醇-乙腈-乙酸铵溶液(体积比为20:10:70)为流动相等度洗脱,采用二级管阵列检测器(DAD)检测,以标准曲线法定量。对乙酰氨基酚在245 nm波长处有最大吸收,其质量浓度在10-400μg/mL范围内与色谱峰面积线性关系良好,线性相关系数大为0.9999。在不同类型空白基质中进行加标回收试验,加标回收率为97.73%-101.00%,测定结果的相对标准偏差为0.04%-0.74%。A method for the determination of acetaminophen in cosmetics by high performance liquid chromatography was established,the suspected results were confirmed and analyzed by high performance liquid chromatography–mass spectrometric.After the samples were extracted with methanol ultrasound,separated by C_(18) column(250 mm×4.6 mm,5μm)eluted,with methanol–0.02 mol/L acetonitrile–ammonium acetate solution(acetic acid was adjusted to pH 4.2,the volume ratio was 20:10:70)as mobile phase under isocratic elution,detected by diode–array–detector(DAD),and quantified by standard curve.The mass concentration of acetaminophen had a good linear relationship with the chromatographic peak area in the range of 10–400μg/mL,the correlation coefficient was 0.9999.Test for recovery was conducted by addition of standard solution to blank sample for different types of cosmetics,and the recoveries rate were in range of 97.73%–101.00%,the relative standard deviations of measurement results were 0.04%–0.74%.

关 键 词：高效液相色谱 化妆品 对乙酰氨基酚 

分 类 号：O657.7[理学—分析化学]