利用染色体片段置换系定位低温影响水稻萌芽期根长和芽长QTL  

Detection of QTL for Root Length and Bud Length at Germination Stage in Low Temperature Using CSSLs in Rice(Oryza sativa)

作　　者：余镁霞 邓浩东 谭景艾 宋贵廷 吴光亮 陈利平 刘睿琦 邹安东 贺浩华[1,2] 边建民 Yu Meixia;Deng Haodong;Tan Jing’ai;Song Guiting;Wu Guangliang;Chen Liping;Liu Ruiqi;Zhou Andong;He Haohua;Bian Jianmin(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding(Jiangxi Agricultural University),Ministry of Education/Jiangxi Province,Nanchang 330045,Jiangxi,China;Jiangxi Super Rice Engineering Technology Research Center,Nanchang 330045,Jiangxi,China)

机构地区：[1]作物生理生态与遗传育种教育部/江西省重点实验室,江西南昌330045 [2]江西省水稻高水平工程研究中心,江西南昌330045

出　　处：《作物杂志》2021年第6期36-45,共10页Crops

基　　金：国家重点研发计划(2016YFD0101800);江西省杰出青年人才资助计划项目(20192BCB23010);江西省自然科学基金(20192ACBL20017);江西省教育厅科学技术研究项目(GJJ170241)。

摘　　要：根和芽的正常生长和发育对保障水稻产量有重要作用。为探究在低温条件下影响幼芽期根长和芽长的QTL,以9311(受体)和日本晴(供体)染色体片段置换系群体为材料,将萌发种子置于15℃低温处理7d,然后在25℃下恢复生长7d,连续测量根长和芽长,以25℃下根长和芽长作为对照,定位低温条件下影响根长和芽长的QTL。15℃条件下,定位到9个根长QTL,贡献率为8.65%-24.35%,其中q RL15T7.2和q RL15T10.3在根生长不同时期被重复检测到;定位到9个影响芽长的QTL,贡献率为1.73%-28.89%,其中q BL15T6、q BL15T7、q BL15T9和q BL15T10被重复检测到。25℃条件下,定位到9个根长QTL,贡献率为1.73%-28.89%,q RL25T7、q RL25T9.3、q RL25T10和q RL25T12在根生长不同时期被重复检测到;定位到5个影响芽长QTL,贡献率为0.58%-38.26%,q BL25T3.3在芽生长不同时期被重复检测到。其中,第2、7、9和10号染色体上4个Bin标记区域存在既控制根长又控制芽长的QTL。通过比较发现,15℃下定位到的根长或芽长QTL与25℃定位到的根长或芽长QTL均不相同,表明低温条件下控制水稻幼芽期根和芽生长的遗传机制可能与正常温度下不同。The growth and development of roots and shoots have an important influence on yield. In order to explore the quantitative trait loci(QTL) affecting root length and bud length in bud bursting period under different temperature treatments, the chromosome segment substitution lines(CSSLs) population of 9311(recipient)/Nipponbare(donor) used as the experimental materials. The germinated seeds were treated at 15℃ for seven days and then recovery grew at 25℃ for seven days. During the recovered period, 10 seeds were randomly selected to measure root length and bud length. After the treatments, the QTL associated with root length and bud length were detected by the CSSLs population. Under 15℃ treatments, nine QTLs affecting root length were detected, the range of contribution rates was 8.65%-24.35%. qRL15 T7.2, and q RL15 T10.3 were repeatedly detected at different periods of root growth;nine QTL affecting the bud length at 15℃ were detected, and the explained phenotypic variations were from 1.73% to 28.89%, qBL15 T6, q BL15 T7, q BL15 T9, and q BL15 T10 were repeatedly detected at different periods of bud growth;Under 25℃ treatments, nine QTL affecting the root length were located, the range of contribution rates was 1.73%-28.89%, qRL25 T7, q RL25 T9.3, q RL25 T10, and qRL25 T12 were repeatedly detected;five QTL affecting bud length were detected, and the contribution rates were 0.58%-38.26%, only qBL25 T3.3 was repeatedly detected at different periods of bud growth. Four recombination bin markers were located on chromosomes 2, 7, 9, 10, also affected different traits at the same temperature or different traits at different temperatures. The results showed that the QTL affecting root length and bud length were different under different treatments. The results suggest that the genetic mechanism of controlling the growth of roots and buds at the bud stage of rice under low temperature may be different from under normal temperature.

关 键 词：水稻 根长 芽长 QTL 染色体片段置换系 

分 类 号：S511[农业科学—作物学]