利用高密度遗传图谱发掘水稻抽穗期新位点  

Identification of New Heading Date QTLs Using High Density Genetic Map in Rice

作　　者：苏代群 陈亮 李锋 武琦 白君杰 邹德堂[2] 王敬国[2] 刘化龙[2] 郑洪亮[2] Su Daiqun;Chen Liang;Li Feng;Wu Qi;Bai Junjie;Zou Detang;Wang Jingguo;Liu Hualong;Zheng Hongliang(Heilongjiang Province Seed Industry Technical Service Center,Harbin 150008,Heilongjiang,China;Key Laboratory of Germplasm Enhancement,Physiology and Ecology of Food Crops in Cold Region,Ministry of Education,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]黑龙江省种业技术服务中心,黑龙江哈尔滨150008 [2]东北农业大学寒地粮食作物种质创新与生理生态教育部重点实验室,黑龙江哈尔滨150030

出　　处：《作物杂志》2021年第6期58-61,共4页Crops

基　　金：黑龙江省“百千万”工程生物育种重大科技专项“优质抗逆水稻新品种选育”(2020ZX16B01)。

摘　　要：水稻抽穗期是决定水稻种植地区及其季节适应性的关键因素,发掘控制水稻抽穗期相关的新主效QTL至关重要。利用包含527个bin标记的高密度遗传连锁图谱,通过靶向测序基因型检测技术对水稻"空育131/小白粳子"衍生的RIL群体进行抽穗期基因型分析。通过对双亲和RIL群体的基本统计分析发现,双亲抽穗期呈极显著差异,表型处于RIL群体范围内,RIL群体有明显的超亲分离现象,符合正态分布。利用IciMapping 4.2软件的完备区间作图法,在水稻第1、3和7号染色体上共检测到4个QTL,其中3个QTL区间内分别含有与抽穗期相关的已知基因Os GI、Hd6和Ghd7,而qHD-3-1是控制水稻抽穗期的新位点。Heading date is the key factor to determine the rice planting area and its seasonal adaptability, so it is very important to find new major QTLs controlling rice heading date. The high-density genetic linkage map with 527 bin markers of the combinant inbred line(RIL) population derived from ’Kongyu 131/Xiaobaijingzi’ was used for genotype analysis of heading date in rice by genotyping by target sequencing(GBTS). Through the basic statistical analysis of the parents and the RIL population, it was found that the parent’s heading date was extremely significantly different, the phenotypes were within the range of the RIL population, and there was an obvious phenomenon of transgressive segregation that conformed to the normal distribution. Four QTLs were detected on chromosomes 1, 3, and 7 of rice using full interval mapping method of IciMapping 4.2. Among them, three QTLs contained known genes OsGI, Hd6 and Ghd7 related to heading stage, respectively. q HD-3-1 was a new QTL controlling the heading date of rice.

关 键 词：水稻 抽穗期 bin标记 QTL 靶向测序基因型检测技术(GBTS) 重组自交系 

分 类 号：S511[农业科学—作物学]