男男性行为者CCR5、CCR2、SDF1、CXCR4基因多态性与HIV-1感染关联性分析  

Correlation analysis of CCR5,CCR2,SDF1,CXCR4 gene polymorphism and HIV-1 infection in MSM

作　　者：蒋红伟 田洪青[2] JIANG Hongwei;TIAN Hongqing(Linyi Center for Disease Control and Prevention,Linyi 276001,China;Shandong Provincial Hospital for Skin Diseases&Shandong Provincial Institute of Dermatology and Venereology,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250022,China)

机构地区：[1]临沂市疾病预防控制中心,山东临沂276001 [2]山东第一医科大学附属皮肤病医院(山东省皮肤病医院),山东省皮肤病性病防治研究所,山东济南250022

出　　处：《中国麻风皮肤病杂志》2022年第2期78-82,共5页China Journal of Leprosy and Skin Diseases

基　　金：山东省医药卫生科技发展计划项目(编号:2017WS640);山东省重点研发计划(编号:重大科技创新工程2018CXGC1203)。

摘　　要：目的:研究男男性行为者(MSM)艾滋病病毒感染相关辅助受体CCR5、CCR2、CXCR4和SDF1基因多态性与HIV-1感染的相关性。方法:收集HIV-1抗体确证阳性者纳入病例组,HIV抗体筛查阴性者作为对照组,应用聚合酶链反应(PCR)和DNA测序法,检测两组MSM样本CCR5基因△32(rs333)和59029A/G(rs1799987)、CCR2、SDF1、CXCR4基因外显子区域的等位基因多态性,分析病例组和对照组基因型分布差异与HIV-1感染的相关性。结果:共收集病例组102例,对照组91例,两组样本CCR5基因rs333位点未检出杂合子和纯合子突变,rs1799987A/G位点突变频率为59.59%,病例组和对照组基因型差异分析显示,rs1799987A/G位点变异可能为影响HIV感染的风险因素[比值比(OR)=2.998,95%可信区间(CI):1.034~8.696];rs1799987A/G、rs1799864、rs1799865、CXCR4、SDF1基因突变频率分别为59.59%、19.95%、26.61%、11.92%和0.26%;CCR2第三外显子测序产物多态性分析发现,病例组和对照组差异有统计学意义(P<0.05),经多因素回归分析未发现与HIV-1感染明显关联。结论:本地区MSM人群未发现CCR5基因△32突变以及对HIV-1感染的保护作用,59029A/G突变频率较高(为59.59%),CCR5、CCR2、SDF1和CXCR4基因多态性与HIV-1感染未发现明显关联。Objective:To study the correlation between HIV-1 infection and gene polymorphisms of CCR5,CCR2,CXCR4 and SDF1 as HIV coreceptors in population of men who have sex with men(MSM)of Han nationality in Shandong Province.Methods:HIV-1 antibody positive cases from MSM were collected in the case group,and HIV antibody negative cases were collected in the control group.The mutations of allelic polymorphisms of△32(rs333)and 59029A/G(rs1799987)of C-C chemokine receptor 5(CCR5),as well as of the gene exon regions of CCR2,SDF1 and CXCR4 were detected by polymerase chain reaction(PCR)and DNA sequencing.The differences in genotype distribution between the case group and the control group were compared,and correlation of the differences with HIV-1 infection was analyzed.Results:There were 102 patients and 91 cases in the case group and control group.Heterozygous and homozygous mutations were not detected at rs333.The genotype difference between the case group and the control group of rs1799987A/G locus variation could be the risk factor of the HIV-1 infection[OR=2.998,95%CI:1.034-8.696].The mutation frequencies of rs1799987A/G,rs1799864,rs1799865 and CXCR4,SDF1 are 59.59%,19.95%,26.61%,11.92%and 0.26%,respectively.There was a significant difference of polymorphism analysis of sequencing products of the third exon of CCR2 between the case group and the control group,which had no correlation with the HIV-1 infection.Conclusion:There is no△32 mutation of CCR5 gene and protective effect on HIV-1 infection among MSM.The allelic mutation frequency of 59029A/G is high(59.59%).There was no correlation between HIV-1 infection and the gene polymorphisms of CCR5,CCR2,SDF1 and CXCR4.

关 键 词：男男性行为 趋化因子受体 艾滋病 基因多态性 

分 类 号：R512.91[医药卫生—内科学]