免疫荧光技术在皮肤活检组织真菌染色中的应用  

Application of immunofluorescent staining method for detection of fungi in dermatopathology

作　　者：陈学超[1] 于永翔[1] 王建文[1] 暴芳芳[1] 周桂芝[1] 陈声利[1] 卢宪梅[1] 刘永霞[1] CHEN Xuechao;YU Yongxiang;WANG Jianwen;BAO Fangfang;ZHOU Guizhi;CHEN Shengli;LU Xianmei;LIU Yongxia(Shandong Provincial Hospital for Skin Diseases&Shandong Provincial Institute of Dermatology and Venereology,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250022,China)

机构地区：[1]山东第一医科大学附属皮肤病医院(山东省皮肤病医院),山东省皮肤病性病防治研究所,济南250022

出　　处：《中国麻风皮肤病杂志》2022年第2期101-103,共3页China Journal of Leprosy and Skin Diseases

基　　金：山东省自然科学基金(编号:ZR2019PH069)。

摘　　要：目的:评价免疫荧光染色法在皮肤活检组织真菌染色中的应用价值。方法:经组织病理和组织培养确诊的43例皮肤真菌病患者对其石蜡包埋切片分别进行免疫荧光染色、PAS染色。结果:43例皮肤真菌病中PAS染色阳性27例(62.8%),免疫荧光染色阳性30例(69.7%),免疫荧光染色法较PAS染色阳性率有所提高,两者差异无统计学意义(P>0.05)。同时两种方法均阴性患者5例,两种方法联合真菌检出阳性率为88.4%。结论:免疫荧光染色是提高皮肤活检组织中真菌检出的重要补充。Objective:To evaluate the application of immunofluorescent staining method in detecting the fungi in skin tissues.Methods:A total of 43 formalin-fixed paraffin-embedded tissues which were indentified as fungal infection by pathology and fungal culture were stained with Calcofluor White and Periodic acid-Schiff(PAS)stain.Results:In 43 cases,27 cases(62.8%)were positive of PAS,30 cases(69.7%)were positive of immunofluorescent staining,with no significant difference(P>0.05).There were 5 patients both negitive of immunofluorescent staining and PAS and the positivity rate was 88.4%of two methods combined.Conclusion:Fluorescent staining is an supplement for detection of fungi in skin tissues.

关 键 词：免疫荧光技术 皮肤真菌病 活检组织 

分 类 号：R756[医药卫生—皮肤病学与性病学]