稻瘟病菌中小G蛋白Rho3的假定互作蛋白MoKin1的功能分析  

Functional Analysis of Mo Kin1 as Putative Substrate of the Small GTPase MoRho3 in Rice Blast Fungus, Magnaporthe oryzae

作　　者：蔡燕 林婕 何金 杨福长 张冬梅[2] 张连虎 王宗华[2,3] CAI Yan;LIN Jie;HE Jin;YANG Fuchang;ZHANG Dongmei;ZHANG Lianhu;WANG Zonghua(School of Agriculture Sciences,Jiangxi Agricultural University,Nanchang,Jiangxi,330045,China;College of Plant Protection,Fujian Agriculture and Forestry University/State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops,Fuzhou,Fujian 350002,China;Institute of Oceanography Minjiang University,Fuzhou,Fujian,350002,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]福建农林大学植物保护学院/福建农林大学闽台作物有害生物生态防控国家重点实验室,福建福州350002 [3]闽江学院海洋研究院,福建福州350002

出　　处：《热带作物学报》2021年第11期3250-3260,共11页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金地区基金项目(No.32060597);江西省自然科学基金青年项目(No.20212BAB215010)。

摘　　要：小G蛋白MoRho3在稻瘟病菌生命活动中具有重要的生物学功能。前期研究表明,MoRho3的缺失对于稻瘟病菌分生孢子的形态建设、附着胞萌发和侵入能力以及对宿主的致病性等表型都存在严重缺陷。为了进一步解析MoRho3在稻瘟病菌中的网络调控途径,通过生物信息学的方法预测了MoRho3的互作蛋白。在众多假定互作蛋白中,蛋白激酶MoKin1与酿酒酵母中KIN1和KIN2是同源蛋白。在酵母细胞中,KIN1和KIN2可以抑制△rho3的表型缺陷。为了验证在稻瘟病菌中MoKin1与MoRho3之间是否存在着相似的调控途径,本研究通过分析MoKin1与同源蛋白的进化关系,序列结构,定位状态以及相关的互作蛋白,揭示了MoKin1的功能特点,为后续病原菌中MoKin1与MoRho3互作机制的研究,以及病原菌致病机制的解析提供了新的思路和方向。Small GTPase MoRho3 plays an important biological role in the rice blast fungus, Magnaporthe oryzae. The preamble research elucidated that the deletion of MoRho3 in this fungus caused serve phenotypic defects, such as abnormal morphology of conidia, reduction of germination and penetration of appressoria and reduced pathogenicity. In order to understand the mechanism of the regulatory pathways of MoRho3, we adopted the bioinformatics to search the putative interacting protein of MoRho3. We found the homologus protein of Saccharomyces cerevisiae KIN1 and KIN2 in the proteins, named MoKin1. Because the over-expression of KIN1 and KIN2 could rescue some phenotypic defects of △rho3 in S. cerevisiae, in order to verify whether there was a similar regulatory pathway between Mokin1 and MoRho3 in M. oryzae, the study revealed the functional characteristics of Mokin1 by analyzing the evolutionary relationship, sequence structure, localization status and related interaction proteins, which would provide a new basis for the further study of the interaction between Mokin1 and MoRho3 and the pathogenic mechanism of M. oryzae.

关 键 词：稻瘟病菌 MoRho3 MoKin1 互作分析 

分 类 号：S432.44[农业科学—植物病理学]