生物法生产D-阿洛酮糖的研究进展  

Progress in biological production of D-psicose

作　　者：黄月园 徐铮[1] 徐虹[1] HUANG Yueyuan;XU Zheng;XU Hong(State Key Laboratory of Materials-Oriented Chemical Engineering,College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室,江苏南京211800

出　　处：《生物加工过程》2021年第6期581-588,共8页Chinese Journal of Bioprocess Engineering

基　　金：江苏省自然科学基金面上项目(BK20201358)。

摘　　要：D-阿洛酮糖(D-psicose或D-allulose)是一种零热量的功能性稀少糖,具有控血糖、保护胰岛、抗氧化等诸多功效。在广泛调研的基础上,本文总结了D-阿洛酮糖的理化性质,生产关键酶D阿洛酮糖3差向异构酶(D-psicose 3-epimerase,DPE)和D-塔格糖3-差向异构酶(D-tagatose 3-epimerase,DTE)的研究进展,探讨了生物法合成D-阿洛酮糖的工艺方法,分子手段改造DPE和DTE的研究成果以及D-阿洛酮糖的分离纯化方法,并就当前D阿洛酮糖的研究现状与发展前景进行了展望。D-psicose(or D-allulose)is a functional rare sugar with zero calorie,and has numerous functions such as controlling blood sugar levels,antioxidation,and protecting islet cells,etc.On the basis of extensive research,we summarize the physicochemical properties of D-psicose and the discovery of D-psicose 3-epimerase(DPE)and D-tagatose 3-epimerase(DTE)that catalyze the production of D-psicose.The biosynthesis technique for D-psicose by using DPE and DTE,as well as the separation and purification of D-psicose are included.The current research progress and future prospect of D-psicose are also discussed.

关 键 词：D-阿洛酮糖 稀少糖 D-塔格糖3差向异构酶 D-阿洛酮糖3差向异构酶 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] Q814[轻工技术与工程—食品科学与工程]