旱区滴灌条件下不同养分组合对叶用枸杞土壤活性碳库及细菌群落的调控效应  

Effects of Different Nutrient Combinations on Soil Fertility and Bacterial Community of Lycium Chinense Mill Under Drip Irrigation in Arid Area

作　　者：王芳[1,2] 沈舒雨[1] 杨柳 林妍敏 南雄雄 张俊华 WANG Fang;SHEN Shu-yu;YANG Liu;LIN Yan-min;NAN Xiong-xiong;ZHANG Jun-hua(School of Geography and Planning,Ningxia University,Yinchuan 750021,China;China-Arab Joint International Research Laboratory for Featured Resources and Environmental Governance in Arid Regions,Yinchuan 750021,China;Ningxia Forestry Institute,Yinchuan 750004,China;National Wolfberry Engineering Research Center,Yinchuan 750004,China;School of Ecology Environment,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学地理科学与规划学院,银川750021 [2]教育部中阿旱区特色资源与环境治理国际合作联合实验室,银川750021 [3]宁夏林业研究院股份有限公司,银川750004 [4]国家林业和草原局枸杞工程技术研究中心,银川750004 [5]宁夏大学生态环境学院,银川750021

出　　处：《节水灌溉》2021年第12期21-29,共9页Water Saving Irrigation

基　　金：宁夏高等学校科学研究项目(优秀青年培育基金项目,NGY2018008);国家自然科学基金项目(41761066,42067022);宁夏自然科学基金项目(2021AAC03528,2020AAC03268)。

摘　　要：为探明干旱区滴灌条件下不同氮磷养分组合对叶用枸杞土壤活性碳库、细菌多样性及群落特征的影响,该研究设置施氮量(40,60和80 mg/L)、施磷量(10,20和30 mg/L)2因素3水平试验,采用高通量测序等方法研究土壤细菌群落特征与土壤环境因子之间的关系。结果表明,滴灌不同氮磷养分组合通过改善土壤养分状况,从而影响叶用枸杞产量。施氮60 mg/L、施磷30 mg/L处理下,N2P3处理下,叶用枸杞产量最高。施氮60 mg/L、施磷10 mg/L,N2P1处理下叶用枸杞根际土壤细菌群落多样性和丰富度表现最佳。不同氮磷配施处理下根际土壤细菌群落相对丰度存在差异,门水平优势类群主要为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、芽孢菌门(Gemmatimonadetes)和厚壁菌门(Firmicutes)等。冗余分析(RDA)和相关性Heatmap图表明,不同处理下土壤细菌群落多样性与有机碳(TOC)、易氧化有机碳(EOC)、水溶性有机碳(DOC)、微生物量碳(MBC)和硝态氮(NO3-N)等环境因子显著相关。综上,滴灌条件下合理氮磷配施能有效改变土壤细菌群落结构,提升土壤肥力,为旱区叶用枸杞养分高效利用和资源可持续发展提供理论依据。Goji berries(Lycium barbarum L.)widely planted in arid to semi-arid regions are a functional resource characterized by a homology of medicine and food.This study explored the effects of different fertilizer combinations on the soil activated carbon pool and the microbial community structure in the L.barbarum soil under drip fertigation in Ningxia,northwestern China.A two-year field experiment was conducted using different levels of drip nitrogen(40,60,and 80 mg/L)and phosphorus(10,20,and 30 mg/L)fertigation.The results showed that the highest average yield was obtained under treatment with 60 mg/L nitrogen plus 30 mg/L phosphorus.The best microbial community structure diversity and abundance of rhizosphere soil was got under treatment with 60 mg/L nitrogen plus 10 mg/L phosphorus.There were differences in the relative abundance of bacterial communities in rhizosphere soil under different treatments.The dominant taxonomic groups at phylum level were Proteobacteria,Actinobacteria,Chloroflexi,Gemmatimonadetes and Firmicutes.RDA and Heatmap analysis showed that the diversity and composition of bacterial community in rhizosphere soil under different treatments were closely related to soil total organic carbon(TOC),easily oxidized organic carbon(EOC),water-soluble organic carbon(DOC),microbial biomass carbon(MBC)and nitrate nitrogen(NO3-N).In conclusion,rational fertilizer combinations under drip fertigation can change soil microbial community structure and improve soil fertility effectively,which provide theoretical basis for nutrient efficient utilization of L.barbarum and resource sustainable development in arid areas.

关 键 词：氮磷养分 叶用枸杞 细菌群落 土壤碳库 

分 类 号：S661.1[农业科学—果树学]