干地榆与鲜地榆煎煮液的体外抗菌性研究  

In vitro antibacterial activity of decoction of dried and fresh Sanguisorba officinalis

作　　者：石宗盛 汤利娇 宋兴贤 薛世聪 陈冬莲 SHI Zong-sheng;TANG Li-jiao;SONG Xing-xian(Qingyuan Hospital of traditional Chinese Medicine Clinical Laboratory,Guangdong Qingyuan 511500)

机构地区：[1]清远市中医院检验科,广东清远511500

出　　处：《医学检验与临床》2021年第11期42-46,共5页Medical Laboratory Science and Clinics

基　　金：广东省中医药局科研项目(20202266)。

摘　　要：目的:研究干地榆与鲜地榆煎煮液的人体外是否具有抗菌性。方法::分别将干鲜地榆煎煮浓缩为1g/mL,再进一步稀释出不同浓度的药液,分别作用于5种不同的标准菌株,并分析干鲜地榆不同浓度下对标准菌株的抑菌性。结果:干鲜地榆煎煮液体外对金黄色葡萄球菌最低抑菌质量浓度为1.5625mg/mL,干鲜地榆煎煮液体外对肠球菌最低抑菌质量浓度为3.125mg/mL,干鲜地榆煎煮液对大肠杆菌ATCC25922、产ESBLs大肠杆菌ATCC35218在100mg/mL,50mg/mL的稀释浓度下有较弱的抑菌活性,干鲜地榆煎煮液对铜绿假单胞菌ATCC27853在100mg/mL稀释浓度下无抑菌活性。结论:干鲜地榆煎煮液对金黄色葡萄球菌、肠球菌具有较强的体外抗菌性,对大肠杆菌、铜绿假单胞菌的体外抗菌性弱。Objective:To study whether the filtrate of dried and fresh Sanguisorba officinalis has antibacterial activity in vitro.Methods:The dried and fresh Sanguisorba officinalis were decocted and concentrated to 1g/ml,and then diluted to different concentrations.Five different standard strains were applied to the drug,and the antibacterial activity of dried and fresh Sanguisorba officinalis against standard strains was analyzed.Results:The minimum inhibitory concentration of dried and fresh Sanguisorba officinalis against Staphylococcus aureus in vitro was 1.5625mg/ml,The minimum inhibitory concentration of dried and fresh Sanguisorba officinalis against Enterococcus in vitro was 3.125mg/ml,The decoction of dried and fresh Sanguisorba officinalis has weak antibacterial activity against Escherichia coli ATCC25922 and ESBLs producing Escherichia coli atcc35218 at the dilution concentrations of 100mg/ml and 50mg/ml,The decoction of dried and fresh Sanguisorba officinalis has no antibacterial activity against Pseudomonas aeruginosa atcc27853 at the dilution concentration of 100mg/ml.Conclusion:The decoction of dried and fresh Sanguisorba officinalis has strong antibacterial activity against Staphylococcus aureus and Enterococcus in vitro,but weak antibacterial activity against Escherichia coli and Pseudomonas aeruginosa in vitro.

关 键 词：干地榆 鲜地榆 体外抗菌 标准菌株 

分 类 号：R28[医药卫生—中药学]