基于酶燃料电池的自供电生物传感器用于MCF-7细胞的定量检测  

A Self-Powered Biosensor Based on Enzyme Fuel Cell for Quantitative Detection of MCF-7 Cells

作　　者：徐庆章 王心颜 丁彩凤[1] Xu Qingzhang;Wang Xinyan;Ding Caifeng(College of Chemistry and Molecular Engineering,Qingdao University of Science&Technology,Qingdao 266042,China)

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《山东化工》2021年第22期43-44,48,共3页Shandong Chemical Industry

基　　金：国家自然科学基金资助项目(21305073)。

摘　　要：本文提出了一种基于酶燃料电池的自供能生物传感器,基于酶燃料电池的自供电生物传感器因其独特的特性在整个检测过程中不需要额外的电源而备受关注,这使得它们具有简单,快速,低成本,抗干扰,易于使用。在这里开发了一种基于酶生物燃料电池的超灵敏自供电生物传感器,用于检测MCF-7细胞。由导电超分子聚合物水凝胶、金纳米颗粒、葡萄糖氧化酶和MCF-7适体构成生物阳极,由聚多巴胺、金纳米颗粒和漆酶构成生物阴极,两电极在含有葡萄糖的缓冲液中构成一个单室的自供电传感器。当阳极通过适体捕获MCF-7细胞时,显着的空间位阻和静电排斥有效地阻止了阳极和阴极表面之间的电子转移,使传感器的开路电压降低,从而实现对MCF-7细胞的定量检测。In this paper,a self-powered biosensor based on enzyme fuel cell is proposed.The self-powered biosensor based on enzyme fuel cell has attracted much attention because of its unique characteristics,which do not need additional power during the whole detection process,which makes them simple,fast,low cost,anti-interference and easy to use.Here,we have developed an ultra-sensitive self-powered biosensor based on enzyme biofuel cells for the detection of MCF-7 cells.The biological anode is composed of conductive supramolecular polymer hydrogel,gold nanoparticles,glucose oxidase and MCF-7 aptamer,and the biological cathode is composed of polydopamine,gold nanoparticles and laccase.The two electrodes form a single-chamber self-powered sensor in a buffer solution containing glucose.When MCF-7 cells were captured by anode through aptamer,the significant steric and electrostatic repulsion effectively prevented the electron transfer between anode and cathode surface,which reduced the open-circuit voltage of the sensor,thus realizing quantitative detection of MCF-7 cells.

关 键 词：酶燃料电池 生物传感器 导电超分子聚合物水凝胶 聚多巴胺 

分 类 号：TQ9[化学工程] TM911.4[电气工程—电力电子与电力传动]