钛金属表面构建介孔氧化硅薄膜载药系统用于BMP-2缓释  

Mesoporous silica membranes on titanium substrates as a drug release system of BMP-2

作　　者：黄芳 毕玮 孙杨 刘玉普[3] 李伟[3] Huang Fang;Bi Wei;Sun Yang;Liu Yupu;Li Wei(Department of Stomatology,Zhongshan Hospital,Fudan University(Xiamen Branch),Xiamen 361015,China;Department of Stomatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Chemistry,Fudan University,Shanghai 200438,China)

机构地区：[1]复旦大学附属中山医院厦门医院口腔科,福建361015 [2]复旦大学附属中山医院口腔科,上海200032 [3]复旦大学化学系,上海200438

出　　处：《国际医药卫生导报》2021年第24期3764-3769,共6页International Medicine and Health Guidance News

基　　金：上海市科研计划项目(18ZR1406300);厦门市科技计划项目(2018ZSXMWK02)。

摘　　要：目的构建钛金属表面介孔氧化硅薄膜(mesoporous silica membranes on titanium substrates,Ti@mSiO2)负载及缓释骨形态发生蛋白-2(bone morphogenetic protein 2,BMP-2)的载药系统。方法利用油水两相法在钛金属表面生长具有垂直孔道的介孔氧化硅薄膜,构建所需的药物载体。使用扫描电子显微镜(scanning electron microscope,SEM)观察材料表面形态及孔径大小;紫外-可见光分光仪测量并对比吸附平衡线性方程,计算得出Ti@mSiO2载体对BMP-2吸附量;间接紫外光分光光度法检测Ti@mSiO2载体的BMP-2缓释量。结果钛金属表面具有厘米尺度上均匀分布的介孔氧化硅薄膜,薄膜具有超薄的厚度,形貌与钛金属表面的微结构一致。由于介孔氧化硅薄膜高的比表面积,明显提高了钛片表面的吸附性能,25℃下10 nm孔径的Ti@mSiO2对BMP-2的吸附量达0.206μg/cm2,是纯钛片的10.5倍。更重要的是,该材料在模拟体液中可实现对BMP-2的缓慢释放,缓释时长由3 h增加到72 h。结论Ti@mSiO2可实现对BMP-2的可控负载及缓释,是促进牙种植体早期骨整合的理想材料。Objective To construct mesoporous silica membranes on titanium substrates(Ti@mSiO2)load-release bone morphogenetic protein 2(BMP-2)as a drug sustained-release system.Methods An oil-water biphase stratification approach was developed to synthesize mesoporous silica membranes which possessed vertical channels on titanium substrates.The scanning electron microscope(SEM)was used to observe the surface morphology of the as-prepared Ti@mSiO2 and the pore size of its mesochannels.The adsorption equilibrium linear equation was measured and compared by ultraviolet-visible spectrometer,and the adsorption amount of Ti@mSiO2 carrier to BMP-2 was calculated.The BMP-2 sustained release of Ti@mSiO2 carrier was determined by indirect ultraviolet spectrophotometry.Results The mesoporous silica membranes grown on titanium substrates(Ti@mSiO2)possessed an uniform mesoporous structure over centimeter scale.The membranes were so thin that the resultant Ti@mSiO2 retained the same size,shape,and surface morphology as the titanium substrates.Due to the enlarged surface areas of the mesochannels,Ti@mSiO2 greatly improved the adsorption performance of titanium surface.The resultant Ti@mSiO2 showed a high adsorption capacity of 0.206μg/cm2 towards BMP-2 when the mesochannels were tuned to 10 nm at 25℃,which was 10.5 times of bare titanium.More importantly,the Ti@mSiO2 showed excellent drug sustained-release behavior,and the release time increased from 3 to 72 hours.Conclusion Due to the good performance of load and sustained-release towards BMP-2,the Ti@mSiO2 is expected to be a promising implant coating material.

关 键 词：钛金属 扫描电子显微镜 模拟体液 纯钛 表面生长 牙种植体 均匀分布 表面形态 

分 类 号：R943[医药卫生—药剂学]