地黄中松果菊苷对谷氨酸诱导的PC-12细胞神经损伤模型的氧化应激和NMDAR1表达的影响  

Effect of Echinacoside Extracted from Dihuang(地黄)on Glutamate-Induced Oxidative Stress and NMDAR1 Expression in PC-12 Cells

作　　者：卢仁睿 王慧慧 张莉[1] 李孟[1] 雷鑫源 郑西雨 冯卫生[1,2] 郑晓珂 Lu Renrui;Wang Huihui;Zhang Li;Li Meng;Lei Xinyuan;Zheng Xiyu;Feng Weisheng;Zheng Xiaoke(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046;Henan Engineering Technology Research Center for Chinese Medicine Development,Zhengzhou 450046)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]河南省中药开发工程技术研究中心,郑州450046

出　　处：《中药药理与临床》2021年第5期45-48,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重点研发计划:中医药现代化研究重点专项:地黄特色中药材产业链关键技术研究(编号:2017YFC1702800);2021年河南省科技攻关项目(编号:212102311106);河南省中医药科学研究专项课题(编号:20-21ZY2151);国家留学基金委访问学者项目(编号:201908410093);河南中医药大学2018年度博士科研基金(编号:BSJJ2018-04)。

摘　　要：目的:观察从地黄中提取的松果菊苷对谷氨酸诱导的PC-12细胞损伤的保护作用及其可能的作用机制。方法:采用谷氨酸诱导PC-12细胞损伤建立神经损伤模型,加入2、5、10μmol/L的松果菊苷,24 h后通过噻唑蓝(MTT)法检测细胞活力,用流式细胞术检测细胞内Ca^(2+)和活性氧(ROS)水平,用In Cell Western法检测谷氨酸受体N-甲基-D-天冬氨酸受体(NMDAR1)水平,用试剂盒检测细胞上清中超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)含量。结果:与正常对照组比较,模型对照组的细胞活力和细胞内SOD活力显著降低(P<0.01),细胞内Ca^(2+)浓度、NMDAR1蛋白表达、ROS、MDA和LDH含量显著升高(P<0.01)。与模型对照组比较,10μmol/L的松果菊苷可以显著提高谷氨酸诱导后PC-12细胞的活力和SOD活力(P<0.01),降低细胞内Ca^(2+)浓度、NMDAR1蛋白表达、ROS、MDA和LDH含量(P<0.01)。结论:松果菊苷可能通过降低细胞内Ca^(2+)的堆积、抑制NMDAR1蛋白的表达及抗氧化作用来改善谷氨酸诱导的PC-12细胞损伤。Objective:To observe the protective effect and mechanism of echinacoside extracted from Dihuang(地黄)against glutamate-induced PC-12 cell injury.Methods:After being damaged by glutamate,the PC-12 cells were treated with 2,5,and 10μmol/L echinacoside.Twenty four hours later,the viability of PC-12 cells was detected by methyl thiazolyl tetrazolium(MTT)assay,and the levels of intracellular Ca^(2+)and reactive oxygen species(ROS)by flow cytometry.The level of glutamate receptor Nmethyl Daspartate receptor(NMDAR)was measured using In Cell Western assay.The levels of superoxide dismutase(SOD),malonaldehyde(MDA),and lactate dehydrogenase(LDH)were determined using the assay kits.Results:Compared with the normal control group,the model control group exhibited significantly diminished cell viability and intracellular SOD activity(P<0.01)and elevated intracellular Ca^(2+),NMDAR1,ROS,MDA,and LDH(P<0.01).Compared with the model control group,10μmol/L echinacoside significantly increased the cell viability and the SOD activity after glutamate induction(P<0.01),and reduced the levels of Ca^(2+),NMDAR1,ROS,MDA,and LDH(P<0.01).Conclusion:Echinacoside effectively alleviates glutamate-induced PC-12 cell damage by reducing the accumulation of intracellular Ca^(2+),inhibiting the expression of NMDAR1,and resisting oxidation.

关 键 词：地黄 松果菊苷 谷氨酸受体 氧化应激 神经损伤 

分 类 号：R285.5[医药卫生—中药学]