蛭龙活血通瘀胶囊对缺血性脑卒中模型大鼠的作用及对脑皮层神经元线粒体的影响  

Effects of Zhilonghuoxuetongyu Capsule in Rat Models of Ischemic Stroke and in Mitochondria of Cerebral Cortex Neurons

作　　者：李亚琴 任维 罗钢 刘梦楠 毛琳慎 韩梅 杨思进 Li Yaqin;Ren Wei;Luo Gang;Liu Mengnan;Mao Linshen;Han Mei;Yang Sijin(Department of Cardiovascular Medicine,Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000;National Traditional Chinese Medicine Clinical Research Base,Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000)

机构地区：[1]西南医科大学附属中医医院心血管内科,泸州646000 [2]国家中医临床研究基地建设单位-西南医科大学附属中医医院,泸州646000

出　　处：《中药药理与临床》2021年第5期119-124,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：2018年四川省科技厅项目(编号:2018SZ0060);泸县人民政府西南医科大学2019年度战略合作成果转移转化培育项目(编号:2019LXXNYKD-01)。

摘　　要：目的:观察蛭龙活血通瘀胶囊对缺血性脑卒中模型大鼠的作用及对脑皮层神经元线粒体结构和功能的影响。方法:除假手术对照组,其余各组均采用改良线栓法制备大鼠大脑中动脉阻塞(Middle cerebral artery occlusion,MCAO)模型,术后24 h评估,造模成功者随机分为模型对照组、蛭龙活血通瘀胶囊0.25、0.5、1 g/kg组和胞磷胆碱钠片0.0625 g/kg组,每组20只。各给药组灌胃给予5%的糖盐水配制的相应药物,假手术对照组和模型对照组给予同体积5%糖盐水,连续灌胃14 d。观察各组大鼠一般状态;神经功能评分和体质量变化;TTC染色观察脑梗死体积;HE染色观察缺血侧病理形态改变;透射电镜观察脑皮层神经元线粒体超微结构;比色法检测脑皮层线粒体三磷酸腺苷(Adenosine triphosphate,ATP)含量和Na^(+)-K^(+)-ATP、Ca^(2+)-Mg^(2+)-ATP酶活力;流式细胞仪检测脑皮层线粒体膜电位。结果:与假手术对照组比较,模型对照组一般状态差,术后第7、14 d神经功能评分显著升高,体质量显著降低,脑梗死体积显著增加(P<0.01),脑组织神经元形态和线粒体结构遭到破坏,脑皮层线粒体ATP含量、Na^(+)-K^(+)-ATP、Ca^(2+)-Mg^(2+)-ATP酶活力以及膜电位显著下降(P<0.01);与模型对照组比较,蛭龙活血通瘀胶囊各剂量组一般状态逐渐变好,体质量明显恢复(P<0.01),神经功能评分和梗死体积均明显改善(P<0.5或P<0.01),脑组织神经元病理形态和线粒体结构损伤有所减轻,膜电位下降的线粒体占比显著减少(P<0.01),蛭龙活血通瘀胶囊0.5、1 g/kg组脑皮层线粒体ATP含量、Na^(+)-K^(+)-ATP、Ca^(2+)-Mg^(2+)-ATP酶活力均明显升高(P<0.5或P<0.01)。结论:蛭龙活血通瘀胶囊可有效减轻缺血性脑卒中大鼠模型神经损伤,其可能是通过提高线粒体ATP含量,维持膜电位稳定等发挥改善作用。Objective:To observe the effect of Zhilonghuoxuetongyu Capsule in ischemic stroke model rats and its effect on the structure and function of cerebral cortical neuron mitochondria.Methods:Except for the sham operation group,rats in the other groups were treated with a modified suture method to establish the middle cerebral artery occlusion(MCAO)rat model,and the evaluation was performed 24 hours after the operation.The successful rat models were randomly divided into the model group,0.25,0.5,1 g/kg Zhilonghuoxuetongyu Capsule groups and 0.0625 g/kg Citicoline sodium tablet group,20 rats in each group.Drugs were prepared in 5%sugar saline,rats were administered with corresponding drugs,while those in the sham operation group and the model group were given the same volume of 5%sugar saline for 14 days.The general state of rats,the nerve function score and the body weight changes were observed.TTC staining was used to observe the cerebral infarction volume.HE staining was used to observe the pathological changes of the ischemic side.Transmission electron microscope was used to observe the ultrastructural changes of cerebral cortex neuron mitochondria,while colorimetric method was used to detect the content of Adenosine triphosphate(ATP)in the cortex mitochondria of rat brains and the activities of Na^(+)-K^(+)-ATP and Ca^(2+)-Mg^(2+)-ATP.Flow cytometry was used to detect the cerebral cortex mitochondrial membrane potential.Results:Compared with the sham operation group,the general statuses of rats were poor in the model group,and the neurological function score was increased on the 7 th and 14 th day after the operation(P<0.01),the body weight was significantly reduced(P<0.01),and the cerebral infarction volume was significantly increased(P<0.01),the neuron morphology and the mitochondrial structures of brain tissues were obviously damaged,the ATP content of cerebral cortex mitochondria,the activities of Na^(+)-K^(+)-ATP,Ca^(2+)-Mg^(2+)-ATPase and the membrane potential were significantly decreased(P<0.01).Compared w

关 键 词：蛭龙活血通瘀胶囊 缺血性脑卒中 线粒体 三磷酸腺苷 膜电位 

分 类 号：R743.3[医药卫生—神经病学与精神病学]