超高效液相色谱法测定复方夏天无片中丹酚酸B含量  

Determination of Salvianolic Acid B in Fufang Xiatianwu Tablets by Ultra Performance Liquid Chromatography

作　　者：刘德鸿 高崇源 郑洋滨 许妍 陈伟康 LIU Dehong;GAO Chongyuan;ZHENG Yangbin;XU Yan;CHEN Weikang(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Provincial Engineering Research Center of Drug and Medical Device Quality,Nanchang Jiangxi 330029,China;School of Pharmacy,Nanchang University,Nanchang Jiangxi 330006,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029 [2]南昌大学药学院,江西南昌330006

出　　处：《药品评价》2021年第21期1300-1302,共3页Drug Evaluation

摘　　要：目的:建立超高效液相色谱(UPLC)法测定复方夏天无片中丹酚酸B的含量。方法:采用Phenomenex-C18色谱柱(2 mm×150 mm,3 μm),以乙腈-0.1%磷酸溶液(21∶79)为流动相;流速为0.3 mL/min;柱温35 ℃;检测波长286 nm。结果:丹酚酸B进样量在1.032 7~41.306 2 μg/mL(r=0.999 7)之间呈良好的线性关系,平均回收率为102.36%,RSD为1.11%(n=6)。结论:UPLC灵敏度高,重现性好,简便易行,可为考察复方夏天无片中丹参的质量控制提供参考。Objective:Ultra performance liquid chromatography (UPLC) method was established for determination of salvianolic acid B in fufang xiatianwu tablets.Methods:Phenomenex C18 column (2 mm×150 mm,3 μm) was used,the mobile phase comprising of acetonitrile-0.1% phosphoric acid solution (21:79),the column temperature was 35 ℃,the velcoity was 0.3 mL/min and the detection wavelength was 286 nm.Results:The linear range of salvianolic acid B was 1.032 7 μg/mL~41.306 2 μg/mL (r=0.999 7),the average recovery was 102.36%,RSD=1.11% (n=6).Conclusion:The method has high sensitivity,good repeatability and simplicity,and can provide a reference for the quality control of radix salviae miltiorrhizae in fufang xiatianwu tablets.

关 键 词：中草药 复方夏天无片 超高效液相色谱法 丹酚酸B 含量测定 

分 类 号：R286.0[医药卫生—中药学]