瑞华麦523 PPO活性及1BL/1RS易位相关基因的分子检测  

Molecular detection of PPO activity and 1BL/1RS translocation related genes in wheat cultivar Ruihuamai 523

作　　者：王歆 金彦刚 夏中华 钱禄巧 郑甲成 WANG Xin;JIN Yan-gang;XIA Zhong-hua;QIAN Lu-qiao;ZHENG Jia-cheng(College of Agronomy,Anhui Science and Technology University,Fengyang,Anhui 233100,China;Jiangsu Ruihua Agricultural Technology Co.,Ltd./Engineering&Technology Research Center for Wheat Commercial Improvement in Jiangsu,Suqian,Jiangsu 223800,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100 [2]江苏瑞华农业科技有限公司/江苏省小麦商业化育种工程技术研究中心,江苏宿迁223800

出　　处：《南方农业学报》2021年第9期2369-2374,共6页Journal of Southern Agriculture

基　　金：国家重点研发计划项目(2017YFD0100702);江苏省农业重大新品种创制项目(PZCZ201706);江苏省科技成果转化专项(BA2017138)。

摘　　要：【目的】了解小麦品种瑞华麦523及其亲本郑麦9023和烟1604的PPO和1BL/1RS易位相关基因的遗传信息,旨在提高优质小麦品种选育的效率。【方法】利用小麦PPO活性基因的分子标记PPO18、PP0-B5、PPO16和PPO29及1BL/1RS易位的分子标记H20,检测小麦品种瑞华麦523及其亲本相关基因的等位变异及来源。【结果】小麦品种瑞华麦523在2AL染色体上PPO等位基因为PPO-A1a基因型,与亲本郑麦9023一样,为高活性PPO,不含亲本烟1604的低活性PPO;在2BL染色体上PPO等位基因为A型,与亲本一致,是高活性PPO;在2DL染色体上PPO等位基因为PPO-D1b基因型,与亲本郑麦9023一样,是一个高活性PPO,不含亲本烟1604的低活性PPO;利用分子标记H20检测表明,瑞华麦523和亲本郑麦9023一样,是一个非1BL/1RS易位系。【结论】瑞华麦523是一个具有高活性PPO、非1BL/1RS易位系的小麦品种,其小麦PPO和1BL/1RS易位相关基因主要来自母本郑麦9023,为该品种的推广应用和培育优质小麦新品种提供理论参考。【Objective】To understand the genetic information of PPO and 1BL/1RS translocation related genes of wheat cultivars Ruihuamai 523 and its parents Zhengmai 9023 and Yan 1604 for the efficiency of high quality wheat breeding.【Method】The allelic variation and origin of the wheat cultivar Ruihuamai 523 and its parent-related genes were detected using the molecular markers PPO18,PP0-B5,PPO16 and PPO29 and 1BL/1RS translocations of wheat PPO activity gene.【Result】The PPO allele of wheat Ruihuamai 523 on chromosome 2AL was the PPO-A1a genotype,like the parent Zhengmai 9023.It was a highly active PPO,lowactive PPO without parent Yan 1604.The PPO allele on chromosome 2BL chromosome was type A,consistent with both parents.It was a highly active PPO.The PPO allele on chromosome 2DL was the PPO-D1b genotype,like the parent Zhengmai 9023,it was a highly active PPO,lowactive PPO without pa rent Yan 1604.Using the molecular marker H20 detection indicated that,Ruihuamai 523 was the same as the parent Zhengmai 9023,it was a non-1BL/1RS translocation line.【Conclusion】The wheat cultivar Ruihuamai 523 is a non 1B/1R translocation line with high activity of PPO.Its genes associated with PPO and 1BL/1RS translocation are mainly derived from female Zhengmai 9023.It provides some references for the variety application and the cultivation of new high quality wheat varieties.

关 键 词：瑞华麦523 多酚氧化酶 易位系 分子标记 

分 类 号：S512.1[农业科学—作物学]