衣康酸二甲酯对非肥胖性糖尿病小鼠胰岛炎影响的实验研究  

Experimental study on the effect of dimethyl itaconate on insulitis in non-obese diabetic mice

作　　者：韩俊永 陈金烟 王坤 金静君 薛士杰 HAN Junyong;CHEN Jinyan;WANG Kun;JIN Jingjun;XUE Shijie(Department of Immunization,Fujian Academy of Medical Science,Fuzhou,Fujian 350001,China)

机构地区：[1]福建省医学科学研究院免疫研究所,福建省医学测试重点实验室,福州350001

出　　处：《福建医药杂志》2021年第6期122-125,共4页Fujian Medical Journal

基　　金：福建省科技厅省属公益类科研基本专项(2019R1011-3);福建省卫健委科研人才培养项目(2018-ZQN-20)。

摘　　要：目的探讨衣康酸二甲酯对非肥胖性糖尿病小鼠(NOD)胰岛炎的影响。方法利用流式细胞仪检测衣康酸二甲酯对RAW 264.7巨噬细胞表型的影响,通过ELISA观察其对RAW 264.7分泌的细胞因子(IL-6、IL-12和TNF-a)的影响,同时将衣康酸二甲酯腹腔注射NOD小鼠,进一步探讨其对胰岛内部炎症的影响。结果衣康酸二甲酯有利于降低巨噬细胞M1/M2比率,使巨噬细胞向M2型转换,同时降低IL-6、IL-12和TNF-α的分泌,50 mg/kg的衣康酸二甲酯使胰岛评分达到了1.115±0.7821。结论衣康酸二甲酯通过M1/M2巨噬细胞表型转化可以缓解NOD小鼠胰岛炎。Objective To investigate the effect of dimethyl itaconate on insulitis in NOD mice.Methods The phenotypic changes of RAW 264.7 macrophages induced by dimethyl itaconate were detected by flow cytometry,and the effect of dimethyl itaconate on the secretion of RAW 264.7 cytokines(IL-6、IL-12 and TNF-α)were observed by ELISA.At the same time,dimethyl itaconate was injected intraperitoneally into NOD mice to further explore its effect on islet inflammation.Results Dimethyl itaconate reduced the M1/M2 ratio of macrophages and converted macrophages to M2 type,and also reduced the secretion of IL-6,IL-12 and TNF-α.The islet score of 50 mg/kg dimethyl itaconate reached 1.115±0.7821.Conclusion Dimethyl itaconate can alleviate insulitis in NOD mice by phenotypic transformation of M1/M2 macrophages.

关 键 词：衣康酸二甲酯 NOD小鼠 巨噬细胞 

分 类 号：R392.6[医药卫生—免疫学]