温敏性羟丁基壳聚糖水凝胶对大鼠全层皮肤缺损创面愈合的影响  

Effects of temperature-sensitive hydroxybutyl chitosan hydrogel on wound healing of full-thickness skin defect in rats

作　　者：陈阿鑫 陈犹白[1] 姜玉峰 韩岩[1] Chen Axin;Chen Youbai;Jiang Yufeng;Han Yan(Department of Plastic and Reconstructive Surgery,the First Medical Center of PLA General Hospital,Beijing 100853,China;Department of Wound Repair Surgery,Special Medical Center of Strategic Support Force,Beijing 100101,China)

机构地区：[1]解放军总医院第一医学中心整形修复科,北京100853 [2]战略支援部队特色医学中心创面修复科,北京100101

出　　处：《中华烧伤杂志》2021年第12期1166-1174,共9页Chinese Journal of Burns

基　　金：解放军总医院扶持基金(ZH19011)。

摘　　要：目的探讨温敏性羟丁基壳聚糖水凝胶对大鼠全层皮肤缺损创面愈合的作用。方法采用实验研究方法。取51只7~10周龄SD大鼠,雌雄不限,在大鼠背部距脊柱约1.0 cm处,制造2个直径2 cm的全层皮肤缺损圆形创面。将大鼠按随机数字表法分为温敏性水凝胶组、凝胶组和空白对照组,每组17只大鼠(34个创面),前2组大鼠创面伤后即刻分别涂抹0.3 mL温敏性羟丁基壳聚糖水凝胶和羧甲基壳聚糖凝胶,空白对照组大鼠创面不行任何处理,3组大鼠创面均外用凡士林油纱。每天换药时观察温敏性水凝胶组大鼠创面温敏性羟丁基壳聚糖水凝胶及凝胶组大鼠创面羧甲基壳聚糖凝胶状态,记录去除凡士林油纱的难易程度。伤后3、7、10、14、21 d,观察3组大鼠创面愈合情况,并计算创面愈合率。伤后3、7、10、14、21 d,每组各取2只大鼠的4个创面组织,行苏木精-伊红染色观察炎症细胞浸润、血管新生、再上皮化情况;Masson染色观察胶原纤维再生和重塑情况,并计算胶原容积分数;酶联免疫吸附测定法检测白细胞介素6(IL-6)、转化生长因子β1(TGF-β1)、基质金属蛋白酶1(MMP-1)的表达。对数据行析因设计方差分析、单因素方差分析及Bonferroni检验。结果伤后换药时,凝胶组大鼠创面羧甲基壳聚糖凝胶呈液态凝胶状,可随体位流动;而温敏性水凝胶组大鼠创面温敏性羟丁基壳聚糖水凝胶呈固态凝胶状,不随体位变化而流动,且分布更均匀;温敏性水凝胶组大鼠创面凡士林油纱较易去除,而其余2组凡士林油纱不易去除。伤后3、7、10、14、21 d,温敏性水凝胶组和凝胶组大鼠创面肉芽组织生长好,未见明显感染;空白对照组伤后3、5 d各有1只大鼠因创面感染而死亡。伤后7、10、14、21 d,温敏性水凝胶组和凝胶组大鼠创面愈合率明显高于空白对照组(P<0.01);伤后10 d,温敏性水凝胶组大鼠创面愈合率明显高于凝胶组(P<0.05)Objective To investigate the effects of temperature-sensitive hydroxybutyl chitosan hydrogel on wound healing of full-thickness skin defect in rats.Methods The experimental research method was used.Fifty-one no matter male or female Sprague-Dawley rats aged 7-10 weeks were selected,and two round full-thickness skin defect wounds with a diameter of 2 cm were created on the back of each rat at a distance about 1.0 cm to the spine.The rats were divided into temperature-sensitive hydrogel group,gel group,and blank control group according to the random number table,with 17 rats and 34 wounds in each group.Wounds of rats in the first two groups were applied respectively with 0.3 mL temperature-sensitive hydroxybutyl chitosan hydrogel and carboxymethyl chitosan hydrogel immediately after injury,and the wounds of rats in blank control group received no treatment.The wounds of rats in the three groups were all covered with vaseline oil gauze.The states of temperature-sensitive hydroxybutyl chitosan hydrogel in wounds of rats in temperature-sensitive hydrogel group and carboxymethyl chitosan hydrogel in wounds of rats in gel group were observed every day when the dressings were changed,and the difficulty of vaseline oil gauze removal was recorded.On the 3rd,7th,10th,14th,and 21st day after injury,the wound healing of rats in the three groups was observed and the wound healing rates were calculated.On the 3rd,7th,10th,14th,and 21st day after injury,tissue from 4 wounds of 2 rats in each group was collected for the following observation and detection.The infiltration of inflammatory cells,angiogenesis,and re-epithelialization were observed by hematoxylin eosin staining.The regeneration and remodeling of collagen fibers were observed by Masson staining,and the collagen volume fraction was calculated.The expressions of interleukin-6(IL-6),transforming growth factorβ1(TGF-β1),and matrix metalloproteinase-1(MMP-1)were detected by enzyme-linked immunosorbent assay method.Data were statistically analyzed with analysis of varianc

关 键 词：伤口愈合 生物敷料 水凝胶 温敏性壳聚糖水凝胶 细胞因子 

分 类 号：R64[医药卫生—外科学] TP212.1[医药卫生—临床医学]