γδT细胞在致死型约氏疟原虫感染BALB/c小鼠中作用  

Role ofγδT cells in lethal Plasmodium yoelii infection in BALB/c mice

作　　者：白排辉 李丹妮 刘军[1] 曹雅明[1] BAI Pai-hui;LI Dan-ni;LIU Jun(Department of Immunology,College of Basic Medical Sciences,China Medical University,Shenyang,Liaoning Province 110122,China;不详)

机构地区：[1]中国医科大学基础医学院,辽宁沈阳110122 [2]中国医科大学附属盛京医院

出　　处：《中国公共卫生》2021年第11期1663-1666,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81871683)。

摘　　要：目的探讨γδT细胞在感染致死型约氏疟原虫(P.y 17XL)的BALB/c小鼠免疫应答中作用及机制。方法6~8周雌性BALB/c小鼠随机分为对照组、P.y 17XL感染组和γδT消除组。小鼠经腹腔注射P.y 17XL感染红细胞1×10;个/只。γδT消除方法为感染前连续2 d腹腔注射TCRγ/δ单抗(150μg/只),对照组和P.y 17XL感染组注射等体积同型对照抗体。动态检测红细胞感染率并观察小鼠生存率。感染后3、5 d,制备脾细胞悬液,流式细胞术检测γδT、DCs和Th1细胞数量;体外培养脾细胞,48 h后收集上清,ELISA检测γ干扰素(IFN-γ)和肿瘤坏死因子α(TNF-α)水平。结果与对照组比较,感染后3、5 d,P.y 17XL感染组小鼠脾脏中γδT细胞数量明显升高(P<0.05),消除组小鼠脾脏中γδT细胞数量明显降低(P<0.01)。感染后5 d,与感染组比较,γδT消除组小鼠脾脏中DC细胞数量及活化水平明显增加,IFN-γ和TNF-α含量明显升高,Th1细胞数量明显增加。结论γδT细胞参与P.y 17XL红内期急性期感染;消除γδT细胞后,Th1型免疫应答代偿性增加。Objective To explore the role and mechanism ofγδT cells in immune response of BALB/c mice infected with the lethal Plasmodium yoelii 17XL(P.y 17XL).Methods Female BALB/c mice at 6-8 weeks of age were randomly divided into a normal control,P.y 17XL infection,and P.y 17XL infection andγδT cell elimination group.The mice were intraperitoneally injected with P.y 17XL to infect 1×10;red blood cells per mouse.The mice ofγδT cell elimination group were intraperitoneally injected with anti-mouse TCRγ/δmonoclonal antibody(150μg/mouse)for two consecutive days before P.y 17XL infection,while the mice of normal control and P.y 17XL infection group were injected with the same volume of isotype control antibody.The infection rate of red blood cells and survival rate of mice were monitored dynamically.The number ofγδT cells,dendritic cells(DCs),and type 1 helper(Th1)cells in spleen cell suspension were detected with flow cytometry at the third and fifth day after P.y 17XL infection.After 48 hours′cultivation in vitro,the supernatant specimens of spleen cell culture in vitro were collected for detections of interferon-γ(IFN-γ)and tumor necrosis factorα(TNF-α)with enzyme-linked immunosorbent assay(ELISA).Results Compared with those of the normal control mice,the number ofγδT cells in spleen tissue of P.y 17XL infected mice increased significantly at the third and fifth day after the infection(both P<0.05);while,the number ofγδT cells in spleen tissue of the mice withγδT cell elimination decreased significantly(both P<0.01).At the fifth day after the P.y 17XL infection,the number and activation of spleen DCs,the number of splenic Th1 cells,and the contents of IFN-γand TNF-αin spleen cell culture supernatants increased significantly in mice withγδT cell elimination in comparison with those in mice of P.y 17XL infection group.ConclusionγδT cells are involved in acute phase infection of P.y 17XL in erythrocytic stage.AfterγδT cell elimination,the Th1 immune response increases as a compensation.

关 键 词：致死型约氏疟原虫(P.y 17XL) ΓΔT细胞 γ干扰素(IFN-γ) 肿瘤坏死因子α(TNF-α) TH1免疫应答 

分 类 号：R392.9[医药卫生—免疫学]