细胞膜融合的PEG修饰脂质体的制备及其体外性质  

Preperation and in vitro characterizations of cell-membrane-fused PEGylated liposomes

作　　者：邓朗 张玉 张奕聪 张志荣[1] DENG Lang;ZHANG Yu;ZHANG Yicong;ZHANG Zhirong(Key IjabonUoTj of Drug Targeting and Drug Delivery of Ministry of Education,West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院靶向药物与释药系统教育部重点实验室,四川成都610041

出　　处：《华西药学杂志》2021年第6期621-624,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:V20A20411)。

摘　　要：目的制备融合胰腺癌细胞PANC-1细胞膜的PEG修饰的脂质体(M-PEG-LIP),并考察其制剂学特征和体外摄取情况。方法结合薄膜水化超声法和挤出法,制备M-PEG-LIP,筛选制备条件,并考察纳米粒的粒径、电位、形态和稳定性。用流式细胞术检测其细胞的摄取效率。结果成功制备了粒径约为130 nm、电位约为-25 mV、具有与脂质体相似的类球形结构、稳定性良好的M-PEG-LIP。胰腺癌细胞对该制剂的摄取同时受到PEG和细胞膜成分的影响。结论 M-PEG-LIP能够结合脂质和细胞膜的特点,具备治疗胰腺癌的潜力。OBJECTIVE To prepare PANC-1 cell-membrane-fused PEGylated liposomes(M-PEG-LIP),and to investigate its prescription properties and uptake efficiency.METHODS The M-PEG-LIP was prepared by combining the thin film hydration ultrasonic method and the extrusion method, and the prescription screening was followed to screen the membrane-to-lipid ratio, the ultrasonic condition and the extrusion condition, and the particle size and potential, morphology and stability of the nanoparticles were investigated.Flow cytometry was used to detect cell uptake efficiency.RESULTS M-PEG-LIP was successfully prepared, with a particle size of about 130 nm, Zeta potential of about-25 mV and spherical structure similar to liposomes.The uptake of M-PEG-LIP by PANC-1 cells was affected by both PEGylation and cell membrane components.CONCLUSION M-PEG-LIP could combine the characteristics of both lipids and cell membranes, processing the potential in treatment of pancreatic cancer.

关 键 词：细胞膜融合 仿生 PEG修饰 脂质体 处方筛选 PANC-1细胞 体外摄取 胰腺癌 

分 类 号：R94[医药卫生—药剂学]