乌甘茶及其饮片与标准汤剂的HPLC指纹图谱相关性研究  

Correlation study on HPLC fingerprint chromatograms of Wugan tea and its decoction pieces and standard decoction

作　　者：张伯言 蒋倩倩 张娇 魏屹[1] ZHANG Boyan;JIANG Qianqian;ZHANG Jiao;WEI Yi(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610000 P.R.China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610000

出　　处：《华西药学杂志》2021年第6期678-682,共5页West China Journal of Pharmaceutical Sciences

基　　金：中藏药大健康产品开发创新团队项目(编号:2018C031);川产道地药材(乌梅)综合开发与区域发展项目(编号:2017ZY006)。

摘　　要：目的建立乌甘茶及其饮片与标准汤剂的HPLC特征图谱,并进行相关性分析。方法通过HPLC法标记15批饮片、标准汤剂、乌甘茶特征图谱的共有峰,指认测定特征峰,测定含量并计算转移率。结果 15批饮片中枸橼酸、绿原酸、甘草苷、桔梗皂苷D、甘草酸的平均含量分别为223.238、0.778、5.558、9.543、36.481 mg·g^(-1);标准汤剂中5种指标成分的平均含量分别为169.841、0.551、3.655、4.091、19.727 mg·g^(-1);乌甘茶中5种成分的平均含量分别为133.668、0.449、2.662、2.571、13.959 mg·g^(-1)。15批饮片、标准汤剂和乌甘茶的指纹图谱均呈现5个共有特征峰,3者对照指纹图谱的相似度为0.90。结论所用方法重复性好,可有效鉴定及控制乌甘茶的质量。OBJECTIVE To establish an HPLC characteristic fingerprint spectrum of Wugan tea and its decoction pieces and standard decoction for correlation analysis.METHODS HPLC method was used to mark the common peaks in the fingerprint spectrum of 15 batches of decoction pieces, standard decoction and Wugan tea, the characteristic peaks were identified, and the content and calculate the transfer rate were determined.RESULTS The average mass fraction of citrate, chlorogenic acid, glycyrrhizin, platycodon saponin D and glycyrrhizin in 15 batches of decoction pieces was 223.238,0.778,5.558,9.543 and 36.481 mg·g^(-1),respectively.The average mass score of the five components in the standard decoction was 169.841,0.551,3.655,4.091 and 19.727 mg·g^(-1),respectively.The average mass score of these five components in Wugan tea was 133.668,0.449,2.662,2.571 and 13.959 mg·g^(-1),respectively.The fingerprint spectrum of 15 batches of decoction pieces, standard decoction and Wugan tea all showed 5 common characteristic peaks, and the similarity of the fingerprints of the three groups was 0.90.CONCLUSION The method used is reproducible and it can effectively identify and control the quality of Wugan tea.

关 键 词：饮片 标准汤剂 乌甘茶 桔梗皂苷D 高效液相色谱法 指纹图谱 相关性 质量控制 慢性咽炎 

分 类 号：R917[医药卫生—药物分析学]