扭果花旗杆DeTIP2;1基因的克隆、亚细胞定位和表达分析  

Cloning,Subcellular Localization and Expression Analysis of DeTIP2;1 Gene from Dontostemon elegans

作　　者：邹丽媛 马薇 李金玉 葛风伟[1] ZOU Li-Yuan;MA Wei;LI Jin-Yu;GE Feng-Wei(College of Life Science,Xinjiang Normal University/Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology/Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang/Key Laboratory of Plant Stress Biology in Arid Land/The Key Discipline Biology,Xinjiang Normal University,Urumqi 830054,China)

机构地区：[1]新疆师范大学生命科学学院/新疆特殊环境物种保护与调控生物实验室/新疆特殊环境物种多样性应用与调控重点实验室/干旱区植物逆境生物学实验室/新疆师范大学校级重点学科生物学学科,乌鲁木齐830054

出　　处：《农业生物技术学报》2021年第12期2279-2288,共10页Journal of Agricultural Biotechnology

基　　金：新疆特殊环境物种保护与调控生物学实验室招标课题基金(XJDX1414-2021-03)。

摘　　要：水通道蛋白(aquaporins,AQPs)在植物生长发育及抵抗非生物胁迫中具有重要的作用。本研究从新疆旱生植物扭果花旗杆(Dontostemon elegans)转录组数据中筛选AQPs家族唯一上调液泡膜内蛋白(D.elegans tonoplast intrinsic protein,DeTIP2;1)基因(GenBank No.MW505902),克隆全长序列,进行生物信息学分析及表达分析,并进行亚细胞定位。结果表明,750 bp的DeTIP2;1编码249个氨基酸,蛋白分子量为24.92 kD,理论等电点为5.30,含有2个高度保守的天冬酰胺-脯氨酸-丙氨酸(asparagine-prolinealanine,NPA)基序。干旱和盐胁迫均能影响DeTIP2;1基因的表达,且该基因的表达具有组织特异性;亚细胞定位显示DeTIP2;1主要定位于液泡膜。上述结果表明,DeTIP2;1可能参与扭果花旗杆根部水分吸收和运输。本研究为干旱区植物资源功能基因的挖掘及筛选提供数据支持。AQPs(aquaporins)play an important role in plant development and abiotic stress resistance.In this study,DeTIP2;1,the only up-regulated gene of AQPs family,was screened from drought transcriptome data of Xinjiang xerophyte Dontostemon elegans.Cloning the full-length sequence,bioinformatics analysis,expression analysis and subcellular localization were carried out.:The results showed that the total length of CDS was 750 bp,encoding 249 amino acids,with a molecular mass of 24.92 kD and a theoretical isoelectric point of 5.30,and contained 2 highly conserved domain of asparagine-proline-alanine(NPA)motifs..Both drought and salt stress could affect the expression of DeTIP2;1,and the expression was tissue specific.Subcellular localization showed that DeTIP2;1 was located in tonoplast.The above results showed that DeTIP2;1 may be involved in water absorption and transportation in the root of D.elegans.This study provides data supports for the mining and screening of functional genes of xerophytic resources in arid areas.

关 键 词：扭果花旗杆 扭果花旗杆液泡膜内蛋白(DeTIP2 1) 生物信息学 亚细胞定位 表达分析 

分 类 号：S3-3[农业科学—农艺学]