浙江省地方品种鸡白血病J亚群感染调查及遗传进化分析  

Investigation on Infection and Genetic Evolution Analysis of Avian Leukemia Subgroup J in the Local Breed Chicken of Zhejiang Province

作　　者：倪征[1] 陈柳[1] 汤傲星 唐井玉 云涛[1] 刘光清[2] 张存[1] NI Zheng;CHEN Liu;TANG Ao-Xing;TANG Jing-Yu;YUN Tao;LIU Guang-Qing;ZHANG Cun(Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《农业生物技术学报》2021年第12期2320-2327,共8页Journal of Agricultural Biotechnology

基　　金：浙江省农业重大技术协同推广项目(2021R22A60D07)。

摘　　要：J亚群禽白血病(subgroup J of avian leukosis)是由肉鸡(Gallus gallus)中分离的一种由J亚群禽白血病病毒(Avian leukosis virus subgroup J,ALV-J)引起的禽白血病的亚群,其临床上主要造成患病禽类的生长和免疫抑制,该病的普遍流行给养禽业带来严重危害。本研究使用ELISA的方法对浙江省杭州光大种禽场的地方品种鸡群鸡白血病J亚群(ALV-J)的感染情况进行了检测,并对阳性样本进行了病毒分离和鉴定。通过扩增分离株的囊膜糖蛋白(envelope glycoprotein 85,GP85)基因,对分离的禽白血病病毒进行了遗传进化分析。研究结果表明,10个地方品种鸡ALV-J阳性检出率差异很大,最低者只有4%,最高者则达到46%。用原代鸡胚成纤维细胞(chicken embryo fibroblasts,CEFs)从检测阳性样本中分离出3株ALVJ(分别命名为ZJ20-1,ZJ20-2和ZJ20-3)。用PCR方法从3个分离株中均扩增出GP85特异性片段,利用DNA STAR软件对分离病毒的GP85基因进行遗传演化分析。结果表明,本研究中分离的ALV-J分离株与参考毒株存在一定的遗传演化关系,可以分为两个拓朴群(G1和G2),分离的3株病毒属于G1群。与国内其他分离株的同源性为88%~98.0%。本研究为进一步深入研究ALV-J在我国鸡场中的流行规律及禽白血病净化提供了参考资料。Subgroup J of avian leukemia is a new subgroup of avian leukemia caused by Avian leukemia virus subgroup J(ALV-J)isolate from broilers(Gallus gallus).Clinically,it mainly causes the growth and immune suppression of diseased poultry,the prevalence of the disease has brought serious harm to the poultry industry.In this study,ELISA method was used to detect the infection of ALV-J in the local breed chicken in GuangDa Poultry Farm in Hangzhou,Zhejiang Province,and the virus was isolated and identified from positive samples.By amplifying the GP85 gene of the isolate,the genetic evolution of the isolated ALV was analyzed.The results showed that the positive rate of ALV-J among 10 local breeds was very different,with the lowest rate being only 4%,and the highest rate being 46%.3 strains of ALV-J(named ZJ20-1,ZJ20-2 and ZJ20-3,respectively)were isolated from the positive samples using primary chicken embryo fibroblasts(CEFs).The GP85 specific fragment was amplified from the 3 isolates by PCR method,and the genetic evolution of GP85 gene was analyzed by DNA STAR software.The results showed that the ALV-J isolates isolated in this study had a certain genetic evolution relationship with the reference strains.The ALV-J isolates could be divided into2 topological groups(G1 and G2),and the 3 isolated strains belonged to G1 group.The homology with other domestic isolates was 88%~98.0%.This study provides a reference for further study on the prevalence of ALVJ in chicken farms in China and the purification of avian leukemia.

关 键 词：J亚群禽白血病病毒(ALV-J) 感染调查 遗传进化分析 

分 类 号：S855.3[农业科学—临床兽医学]