灰葡萄孢MYB转录因子家族基因的鉴定及表达分析  

Identification and Expression Analysis of Transcription Factor MYB Family Genes in Botrytis cinerea

作　　者：朱倩洁 朱自平[3] 于新月 张强[2] 曹宏哲 邢继红[1,2] 张康 董金皋[1,2] ZHU Qian-Jie;ZHU Zi-Ping;YU Xin-Yue;ZHANG Qiang;CAO Hong-Zhe;XING Ji-Hong;ZHANG Kang;DONG Jin-Gao(State Key Laboratory of North China Crop Improvement and Regulation,Hebei Agricultural University,Baoding 071000,China;Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China;Langfang Academy of Agriculture and Forestry Science,Langfang 065000,China)

机构地区：[1]河北农业大学省部共建华北作物改良与调控国家重点实验室,保定071000 [2]河北农业大学河北省植物生理与分子病理学重点实验室,保定071000 [3]廊坊市农林科学院,廊坊065000

出　　处：《农业生物技术学报》2021年第12期2355-2364,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31972217);河北省自然科学基金(C2018204045);河北农业大学科研启动项目(ZD201721)。

摘　　要：MYB(v-myb avian myeloblastosis viral oncogene homolog)转录因子广泛存在于植物病原真菌中,在真菌的生长发育和胁迫响应中发挥重要功能。为了解灰葡萄孢(Botrytis cinerea)MYB转录因子家族的功能,本研究利用生物信息学方法对灰葡萄孢MYB转录因子家族进行系统进化分析、保守结构域分析、分生孢子发育时期和侵染时期的表达规律分析;利用qPCR技术,检测灰葡萄孢MYB家族基因在NaCl和H2O2处理后的表达变化。结果显示,灰葡萄孢中包含22个MYB转录因子家族基因,系统进化分析将其分为6个亚家族,分别是MYB、MYB-Hox、Zn-ZZ-MYB、DEXDc-HELICc-MYB、HAS-MYB和BAH-PND-MYB-Zn-GATA;MYB家族基因在分生孢子的不同发育时期和侵染时期呈现不同的表达规律,在NaCl和H_(2)O_(2)处理后出现不同的表达变化趋势,提示MYB家族基因在灰葡萄孢生长发育、侵染植物以及胁迫应答过程中可能具有重要的功能。本研究为深入探讨MYB转录因子家族的基因功能和灰葡萄孢的防治提供基础资料。MYB(v-myb avian myeloblastosis viral oncogene homolog)transcription factors are widely present in plant pathogenic fungi,and play a variety of roles in the growth,development and stress response of fungi.In order to understand the function of the MYB transcription factor family of Botrytis cinerea,this study used bioinformatics methods to conduct phylogenetic analysis,conserved domain analysis,and analysis of the MYB transcription factor family in B.cinerea.Analysis of the expression regularity during the infection period;and using qPCR technology to detect the expression of the MYB family genes of B.cinerea after NaCl and H2O2 treatment.The results showed that there were 22 MYB transcription factor family genes in Botrytis cinerea,and phylogenetic analysis divided them into 6 subfamilies,namely MYB,MYB-Hox,Zn-ZZ-MYB,DEXDc-HELICc-MYB,HAS-MYB and BAH-PND-MYB-Zn-GATA;MYB family genes showed different expression patterns at different developmental stages and infection stages of conidia,and different expression patterns after NaCl and H_(2)O_(2) treatments,indicating that the genes of MYB family of B.cinerea might play an important role in the growth and development of the pathogen,the process of infection and responding to stress.The present study provides basic material for studying the gene function of the MYB transcription factor family and the control of B.cinerea.

关 键 词：灰葡萄孢 MYB(v-myb avian myeloblastosis viral oncogene homolog) 转录因子 生物信息学分析 基因表达 

分 类 号：S432.44[农业科学—植物病理学]