生物燃料颗粒物暴露对大鼠肺泡巨噬细胞的络氨酸激酶抑制剂受体和肺表面活性蛋白D表达的影响  

Chronic exposure of biomass ambient particulate matter induces signal-regulatory proteinα(SIRPα)and surfactant protein D expression in rat alveolar macrophages

作　　者：王慎临 李冰[2] 冉丕鑫[3] Wang Shenlin;Li Bing;Ran Pixin(Department of Respiratory Medicine,the First Affiliated Hospital of Northwest University for Nationalities,Ningxia Hui Autonomous Region People's Hospital,Yinchuan 750021,China;State Key Laboratory of Respiratory Diseases,National Clinical Research Center for Respiratory Diseases,Guangzhou Institute of Respiratory Health,Guangzhou Medical University,Guangzhou 510120,China)

机构地区：[1]西北民族大学附属第一医院,宁夏回族自治区人民医院,银川750021 [2]广州医科大学生命科学院,510120 [3]广州医科大学呼吸疾病国家重点实验室,广州呼吸健康研究所,510120

出　　处：《中华结核和呼吸杂志》2021年第12期1090-1096,共7页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金(81970045);宁夏自然科学基金项目(2019AAC03305);西北民族大学中央高校基本科研业务费专项资金项目(31920180090)。

摘　　要：目的观察生物燃料颗粒物(BMF)对肺泡巨噬细胞(AM)的络氨酸激酶抑制剂受体(SIRPα)、肺表面活性蛋白(SP)A和D的表达及吞噬功能的影响。方法将72只雄性SD大鼠使用随机数字表分为BMF组和清洁空气组,通过蛋白印迹法和免疫荧光检测BMF暴露4 d、1及6个月大鼠AM的SIRPα、SPA和SPD的表达,采用荧光标记金黄色葡萄糖球菌和铜绿假单胞菌,检测三个时间点大鼠AM吞噬细菌的能力。结果 BMF暴露4 d,SIRPα和SPD的蛋白与清洁空气组相比,差异无统计学意义(P>0.05);BMF暴露1个月SIRPα和SPD的蛋白相对表达量为(1.73±0.64)和(2.01±0.78),BMF暴露6个月SIRPα和SPD的蛋白相对表达量为(1.49±0.28)和(1.48±0.34),均高于清洁空气组(P<0.05)。BMF暴露1个月大鼠AM吞噬金黄色葡萄球菌和铜绿假单胞菌的MFI相对比为(0.56±0.16)和(0.80±0.09),BMF暴露6个月大鼠AM吞噬金黄色葡萄球菌和铜绿假单胞菌的MFI相对比为(0.67±0.11)和(0.76±0.16),均低于清洁空气组(P<0.05)。结论 BMF诱导AM的SIRPα和SPD的表达上调,抑制AM的吞噬功能。Objective To observe the expression of signal-regulatory proteinα(SIRPα),surfactant protein(SP)A,and SPD by,and the phagocytic function of alveolar macrophages(AMs)in the rats exposed to biomass ambient particulate matter(BMF).Methods Seventy-two male SD rats were randomly divided into BMF group and clean air group.Protein levels of SIRPα,SPA,and SPD in AMs were determined by Western blotting and immunofluorescent assay after 4 days,1 month,and 6 months of BMF exposure.Fluorescent labeled Glucose aureus and Pseudomonas aeruginosa were used to detect the phagocytic ability of AMs in rats at three time points.Results After 4 days of BMF exposure,there was no significant difference in the protein levels of SIRPαand SPD compared with the clean air group(P>0.05).The relative levels of SIRPαand SPD were(1.73±0.64)and(2.01±0.78)at 1 month of BMF exposure,and those at 6 months of BMF exposure were(1.49±0.28)and(1.48±0.34),both of which were higher than those in the clean air group(P<0.05).The relative level of Staphylococcus aureus median fluorescence intensity(MFI)and Pseudomonas aeruginosa MFI at 1 month BMF group were(0.56±0.16)and(0.80±0.09),and those at 6 months BMF group were(0.67±0.11)and(0.76±0.16),both of which were lower than those in clean air group,and the difference was statistically significant(P<0.05).Conclusions BMF induces upregulation of SIRPαand SPD in AMs and inhibits the phagocytosis of AMs.

关 键 词：生物燃料颗粒物 肺泡巨噬细胞 络氨酸激酶抑制剂受体 吞噬功能 

分 类 号：R563.9[医药卫生—呼吸系统]