Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells  

作　　者：Chenyi An Wei Chen 

机构地区：[1]Department of Cell Biology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine,Zhejiang University,Hangzhou 310058,China [2]Key Laboratory for Biomedical Engineering of Ministry of Education and State Key Laboratory for Modern Optical Instrumentation,Zhejiang University,Hangzhou 310058,China [3]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang University,Hangzhou 310058,China

出　　处：《Biophysics Reports》2021年第5期377-383,共7页生物物理学报（英文版）

基　　金：This work was supported by grants from the National Basic Research Program of China(2015CB910800 and 2017ZX10203205);the National Science Foundation of China(31522021 and 31971237);the Fundamental Research Funds for the Central Universities(2015XZZX004-32);China Postdoctoral Science Foundation(2020M681834)。

摘　　要：Complex physical cues including two-dimensional membrane environment,dynamic mechanical force,and bioelectric activity inevitably affect membrane receptor functions.Multiplexed single-molecule force spectroscopy(SMFS)techniques with the capability of live-cell measurements are essential to systemically dissect receptor’s functions under complex biophysical regulation.In this review,we summarize recent progress of live-cell based SMFS techniques and specifically focus on the progress of SMFS on the biomembrane force probe with enhanced mechanical stability and multiplexed capability of fluorescence imaging.We further suggest the necessity of developing multiplexed SMFS techniques with simultaneous bioelectric regulation capability to investigate membrane potential regulated membrane receptor functions.These state-of-art multiplexed SMFS techniques will dissect membrane receptors functions in a systematic biophysical angle,resolving the biochemical,biomechanical and bioelectrical regulatory mechanisms in physiologically relevant conditions.

关 键 词：Multiplexed SMFS techniques Membrane receptor Biophysical regulation Live cell 

分 类 号：Q27[生物学—细胞生物学]