广州市2010―2019年4型登革热病例流行特征及其病毒E基因  

Epidemiology of dengue virus serotype 4 cases and E gene analysis of dengue virus in Guangzhou Province from 2010 to 2019

作　　者：蒋力云[1] 刘远[1] 苏文哲[1] 罗雷[1] 曹毅敏[1] 狄飚[1] 吴新伟[1] 张周斌[1] JIANG Li-yun;LIU Yuan;SU Wen-zhe;LUO Lei;CAO Yi-min;DI Biao;WU Xin-wei;ZHANG Zhou-bin(Department of Virology and Immunology,Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China)

机构地区：[1]广州市疾病预防控制中心病毒免疫部,广州510440

出　　处：《中华疾病控制杂志》2021年第11期1360-1364,共5页Chinese Journal of Disease Control & Prevention

基　　金：广州市科技计划(202002030117);广州市卫生健康科技项目(20191A011059);广州市重点实验室基础研究计划(2021BRP004);广州市医学重点学科建设项目(2021-2023-11)。

摘　　要：目的了解2010―2019年广州市4型登革热病例的流行病学特征和4型登革病毒的分子生物学特征。方法2010―2019年,收集广州市登革热病例相关资料及血清标本,使用RT-PCR法检测血清标本并分型,对4型登革热病例标本进行登革病毒包膜(envelope,E)蛋白基因序列测定,并进行进化树构建及基因重组分析。结果2010―2019年,广州市共报告43174例登革热病例,其中23例为4型登革热病例。4型登革热病例仅分布在5个区,曾在2010年出现本地流行。广州市的4型登革热输入病例主要来自东南亚国家,病毒E基因序列与东南亚国家的序列有较高相似性,病毒基因型归属于印度尼西亚基因型和东南亚基因型,未检测到基因重组。结论应加强对广州市4型登革热病例流行状况和输入病例的监测和关注,并进一步加强对东南亚回国人员登革热常识和个人防护的健康宣传。某些区需要预防出现4型登革病毒的扩散和蔓延。登革病毒基因型转换并未引起登革热在广州市流行强度的变化,有待进一步的观察和研究其是否会发生重组。Objective Investigate epidemiology of dengue virus serotype 4 cases,and molecular biological characteristics of dengue virus serotype 4 circulating in Guangzhou from 2010 to 2019.Methods Information and serum samples of dengue patients were collected.Serotypes were confirmed by real-time PCR.Envelope(E)gene of dengue virus serotype 4 was sequenced.Phylogenetic tree was constructed,and recombination was analyzed.Results A total of 43174 dengue cases were reported in Guangzhou between 2010 and 2019,of which 23 cases were confirmed as serotype 4.Serotype 4 cases can only be detected in 5 districts.An endemic of dengue virus serotype 4 broke out in 2010.The imported cases were mainly from Southeast Asia.Sequences of E gene had a high similarity with sequences from Southeast Asia.Sequences acquired in Guangzhou clustered into Indonesia genotype and Southeast Asia genotype.No recombination has been detected.Conclusions Supervision on dengue virus serotype 4 cases and imported cases should be enforced.Conduct propaganda of knowledge about dengue fever and personal protection should be further done among people coming back from Southeast Asia.Dengue virus serotype 4 must be prevented from spreading,especially in some districts.The genotype shift didn’t cause any change of epidemic.Although there was no sign of recombination,further research is needed.

关 键 词：登革热 流行特征 E基因 基因进化树 

分 类 号：R512.8[医药卫生—内科学] R181.3[医药卫生—临床医学]