苯酚烧伤大鼠的体内毒物分布及代谢  

Distribution and metabolism of toxicants in rats with phenol burn

作　　者：滕力群 廖欣[1] 李卫[1] Teng Liqun;Liao Xin;Li Wei(Department of Burns Nuclear and Chemical Accident Rescue&Treatment Center,Jinshan Hospital Affiliated to Fudan University,Shanghai 201508,China)

机构地区：[1]复旦大学附属金山医院核化学伤害应急救治中心烧伤科,上海201508

出　　处：《中华劳动卫生职业病杂志》2021年第11期859-861,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的研究苯酚烧伤后大鼠体内的毒物分布及代谢情况。方法于2019年2月,将SPF级健康SD雄性大鼠按6 mg/kg苯酚经皮染毒制作大鼠5%体表面积烧伤模型,采用高效液相色谱法分别测定0.25、0.75、2、4、8、16和32 h后大鼠血浆和肾脏组织内苯酚含量。通过DAS 2.0软件计算苯酚代谢动力学参数,并对其肾脏靶向性进行评价。结果大鼠苯酚烧伤后0-8 h的血药浓度-时间曲线下面积(AUC_(0-8))为(28.741±6.485)μg/ml·h,0至无限时间的血药浓度-时间曲线下面积(AUC_(0-∞))为(30.354±6.424)μg/ml·h,半衰期(t_(1/2))为(2.111±0.632)h,峰浓度(C_(max))为(16.287±4.870)μg/ml,平均滞留时间(MRT)为(1.854±0.148)h;大鼠肾脏的靶向效率(DTE)为2.91。结论苯酚烧伤大鼠苯酚经皮肤吸收快,消除快,并且清除率高、MRT短、物质蓄积性弱,苯酚对肾脏具有相对明显的选择性。Objective To study the distribution and metabolism of toxicants in rats after phenol burn.Methods In February 2019,SPF-grade healthy SD male rats were transdermally exposed to 6 mg/kg phenol to create a 5% body surface burn model of rats.High performance liquid chromatography was used to determine phenol content in rat plasma and kidney tissues after 0.25,0.75,2,4,8,16,and 32 h,respectively.The kinetic parameters of phenol were calculated by DAS 2.0 software,and the kidney targeting of phenol was evaluated.Results The area under the blood concentration-time curve at 0-8 h(AUC_(0-8))of the rat after phenol burn was(28.741±6.485)μg/ml·h,and the area under the blood concentration-time curve from 0 to infinite time(AUC_(0-∞))was(30.354±6.424)μg/ml·h,half-life(t_(1/2))was(2.111±0.632)h,peak concentration(Cmax)was(16.287±4.870)μg/ml,mean residence time(MRT)was(1.854±0.148)h.The target efficiency(DTE)of rat kidney was 2.91.Conclusion Phenol burn rats have fast percutaneous absorption,rapid elimination of phenol,and have high clearance rate,short MRT,and weak substance accumulation.Phenol has relatively obvious selectivity to the kidneys.

关 键 词：苯酚 烧伤 大鼠 色谱法 高效液相 毒动学 毒物动力学 

分 类 号：R644[医药卫生—外科学]