^(60)Co-γ射线辐射水稻小RNA测序变异分析  

Analysis of Small RNA Sequencing Variation in Rice Irradiated by^(60)Co-γ Ray

作　　者：张莉 张勇[1] 雷星宇 张渊海 张逸妍[1] 彭选明[1] 杨震[1] ZHANG Li;ZHANG Yong;LEI Xingyu;ZHANG Yuanhai;ZHANG Yiyan;PENG Xuanming;YANG Zhen(Hunan Institute of Nuclear Agriculture Sciences and Space Mutagenesis Breeding,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125)

机构地区：[1]湖南省农业科学院核农学与航天育种研究所,湖南长沙410125

出　　处：《核农学报》2021年第11期2441-2450,共10页Journal of Nuclear Agricultural Sciences

基　　金：湖南省农业科技创新资金项目(2021CX38);国家十三五重点研发专项项目(2016YFD0102103);湖南省自然科学资金项目(2020JJ5287);国家自然科学资金(11205055)。

摘　　要：为探讨γ射线辐射引起水稻损伤效应的小RNA变异,采用0、300和400 Gy的^(60)Co-γ射线辐射处理水稻高粳糯种子,利用Illumina HiSeq^(TM)2500高通量测序平台对辐射后的高粳糯三叶期叶片进行小RNA测序。结果显示,0 Gy(对照)组共获得7395578个小RNA,254个已知miRNA成熟序列,26个新miRNA成熟序列;300 Gy剂量组(Gy3)共获得10315701个小RNA,265个已知miRNA成熟序列,29个新miRNA成熟序列;400 Gy剂量组(Gy4)共获得6469869个小RNA,261个已知miRNA成熟序列,29个新miRNA成熟序列。GO富集分析显示,Gy4 vs CK组差异miRNAs对应的候选基因富集到生物过程、细胞组分和分子功能三大类共58个小类,其中涉及分子功能的基因最多。Gy3 vs Gy4组差异miRNAs对应的候选基因富集到生物过程和分子功能两大类共7个小类,以离子结合、过渡金属离子结合和氧化还原酶活性占主要比例。KEGG富集分析显示前20条显著富集途径,Gy3 vs CK组差异miRNAs对应的候选基因富集到植物病原互作最高,富集最显著的是植物昼夜节律。Gy4 vs CK组差异miRNAs对应的候选基因组富集到代谢途径最高,富集最显著的是甘油磷脂代谢和醚脂类代谢,Gy3 vs Gy4组差异miRNAs对应的候选基因富集到甘油磷脂代谢、真核生物的核糖体发生和内吞作用三个途径最高,富集最显著的是醚脂类代谢。本研究从RNA水平为辐射诱变水稻引起当代的苗期损伤效应提供了新见解。In order to research the small RNA variation of damage effect in rice caused by the γ-ray irradiation, radiation doses of 0, 300 and 400 Gy were applied to the rice seeds of Gaogengnuo, a small transcriptome was sequenced by using Ilumina HiSeq^(TM)2500 with three leaf stage seedling of irradiated GaoGengNuo. Result showed that total of 7 395 578 small RNAs, including 254 known miRNAs and 26 novel miRNAs were identified in 0(CK) group;10 315 701 small RNAs, 265 known miRNAs and containing 269 novel miRNAs were identified in the 300 Gy(Gy3) group;6 469 869 small RNAs, containing 261 known miRNAs and 29 novel miRNAs were indentified in 400 Gy(Gy4) group. By Go analysis, the corresponding genes of differentially expressed miRNAs in Gy4 vs CK group were classified into 58 terms of three main GO categories, with the largest proportion of regions performing molecular processes;the differentially expressed miRNAs in Gy3 vs Gy4 group were classified into 7 terms of “biological process” and “molecular function” two main GO categories, among which ion binding, transition metal ion binding and oxidoreductase activity were the three most GO terms. Through analyzing the most significant enrichment 20 pathways with REGG pathway analysis, the corresponding genes of differentially expressed miRNAs in Gy3 vs CK group were majorly involved in plant pathogen interaction pathway, and the most significant pathway was plant circadian rhythm pathways;In Gy4 vs CK group, the highest number of genes were enriched in metabolic pathway, and the most significant pathway were glycerol phospholipids metabolism and ether lipid metabolism;In Gy3 vs Gy4 group, the highest number of genes were enriched in glycerol phospholipid metabolism, ribosome biogenesis in eukaryotes and endocytosis, and the most significant enrichment was ether lipid metabolism. By analyzing the variations of RNA level, it provides a new pathway to study the damage effect of rice seedling by irradiation.

关 键 词：水稻 Γ射线 损伤效应 小RNA GO富集分析 KEGG分析 

分 类 号：S511[农业科学—作物学]