Study on Quality Standard of Tibetan Medicine Corydalis dasyptera Maxim.  

作　　者：Yi SHI Yunfeng HAN Xi TANG Jie GAO Lin PAN Yi ZHANG Xianrong LAI Duojie LONGZHU 

机构地区：[1]School of Ethnic Medicine, Chengdu University of Chinese Medicine, Chengdu 611137, China [2]College of Life Sciences, Qinghai Normal University, Xining 810008, China

出　　处：《Medicinal Plant》2021年第5期28-32,共5页药用植物：英文版

基　　金：Chunhui Project—Identification of the Source of Tibetan Medicine C.dasyptera Maxim.

摘　　要：[Objectives]To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim.[Methods]According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia,8 batches of C.dasyptera Maxim.from different habitats were studied by character identification,microscopic identification and TLC identification.The content of water,total ash,acid-insoluble ash and alcohol-soluble extract was determined,and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC).[Results]The properties and microscopic characteristics of C.dasyptera Maxim.were determined.The TLC characteristic spots of the medicinal materials were clear,the degree of separation was good,and the specificity was strong.Both the test sample and the control sample showed the same yellow-green spots in the corresponding position.It was tentatively determined that the water content of C.dasyptera Maxim.should not exceed 14.0%,the total ash content should not exceed 14.0%,the acid-insoluble ash content should not exceed 3.0%,and the alcohol-soluble extract content should not be less than 18.0%.There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40μg·10-3(r=0.9996).The average recovery rate was 98.47%and the RSD was 1.21%(n=6).The content of protopine in 8 batches of C.dasyptera Maxim.was 0.023%-0.093%.[Conclusions]The established quality research method is simple,stable and reliable,and can be used for the quality control of C.dasyptera Maxim.

关 键 词：Corydalis dasyptera Maxim. Microscopic identification TLC identification Content determination 

分 类 号：R28[医药卫生—中药学]