三链核酸分子探针的稳定性及其生物传感应用  

Stability of triplex nucleic acid molecular probe and its biological sensing application

作　　者：王焕翔 潘松兰 杨华 薛倩 邓祥熙 孙芸琳 赵聪慧 邹振 Wang Huan-xiang;Pan Song-lan;Yang Hua;Xue Qian;Deng Xiang-xi;Sun Yun-lin;Zhao Cong-hui;Zhou Zhen(Hunan Provincial Key Laboratory of Cytochemistry,School of Chemistry and Food Engineering,Changsha University of Science and Technology,Changsha 410114,China)

机构地区：[1]长沙理工大学化学与食品工程学院,细胞化学湖南省重点实验室,湖南长沙410114

出　　处：《化学传感器》2020年第4期26-33,共8页Chemical Sensors

基　　金：国家自然科学基金(21705010,21735001,21605008);湖南省教育厅优秀青年项目(20B032);湖南省研究生创新项目(CX20200890)。

摘　　要：由于独特的可设计性和分子识别能力,核酸分子探针已被广泛应用于生物传感领域。DNA三链结构是继Watson和Crick等发现DNA双螺旋结构后发现的一种特殊结构。它是由第三条寡聚核酸链与DNA双螺旋结构通过Hoogsteen氢键杂交形成的三股螺旋DNA结构。由于第三条核酸链的引入,三链核酸分子探针在信号输出,信号放大和信号编码方面具有操控性,拓展了核酸分子探针在生物传感中的功能应用。该文讨论了影响三链核酸稳定性的主要因素,综述了三链核酸分子探针在生物传感领域的功能应用。Due to unique excellent designable property and specific molecular recognition ability,nucleic acid molecular probes have been widely used in biosensing.The DNA triplex structure is another special structure after the discovery of DNA double helix structure by Watson and Crick.Triple-helix DNA structure is formed by the hybridization between the third oligo-nucleic acid strand and the DNA double helix structure through the Hoogsteen hydrogen bond.Thanks to the introduction of the third nucleic acid strand,the triplex nucleic acid molecular probes have prominent advantage in the signal output,signal amplification and signal coding,which expands the application of the nucleic acid molecular probe in biosensing.This article discusses the main factors affecting the stability of triplex nucleic acid,and summarizes the functional applications of triplex nucleic acid molecular probes in the field of biosensing.

关 键 词：三链核酸 生物传感 核酸分子探针 稳定性 

分 类 号：Q503[生物学—生物化学] TP212.3[自动化与计算机技术—检测技术与自动化装置]