基于金纳米颗粒/共价有机骨架COF-TpPa-1无标记型C-反应蛋白免疫传感器的研制  

Study on C-reactive protein immunosensor based on Au NPs/covalent organic framework COF-TpPa-1

作　　者：白茹燕 张坤蕾 胡蓉[2] 杨云慧[2] Bai Ru-yan;Zhang Kun-lei;Hu Rong;Yang Yun-hui(Kunming Medical University,Hai Yuan College,Kunming 650000,China;College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]昆明医科大学海源学院,云南昆明650000 [2]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学传感器》2020年第4期43-51,共9页Chemical Sensors

基　　金：国家自然科学基金资助项目(No.21765026,21465026)。

摘　　要：采用高温溶剂热法合成了共价有机框架材料(COF-TpPa-1),通过还原法将金纳米颗粒负载到COF-TpPa-1材料上,制得了Au NPs/COF-TpPa-1纳米材料,并将其作为固定基质来固定CRP抗体,制备了无标记型C-反应蛋白(CRP)免疫传感器。修饰在电极上的Au NPs/COF-TpPa-1可直接催化对硝基苯酚的还原,当CRP抗体与抗原结合后,阻碍了电极表面的电子传递速率,使得检测电流减小,通过电流与抗原浓度之间的关系,实现对CRP的检测。线性范围为0.01~150 ng/mL,检测下限为3 pg/mL,相关系数为0.9980。该传感器检测下限低,灵敏度高,方法简便。COF-TpPa-1 was prepared by high-temperature solvothermal method.Au NPs/COF-TpPa-1 composite materials were prepared by loading the gold nanoparticles onto the COF-TpPa-1 material by reduction method.Au NPs/COF-TpPa-1 were modified onto the glassy carbon electrode to immobilize CRP antibody and construct a label-free immunosensor for CRP.Au NPs/COF-TpPa-1 modified on the electrode can directly catalyze the reduction of p-nitrophenol.The binding of CRP antibody and CRP antigen can hinder the transfer rate of redox probe p-nitrophenol on the surface of electrode,leading to decrease of response current signal of p-nitrophenol.There is a linear relationship between signal and CRP antigen concentration within 0.01 to 150 ng/mL with detection limit of 3 pg/mL.The correlation coefficient was 0.9980.This sensor is simple method with low detecting limit and high sensitivity.

关 键 词：C-反应蛋白(CRP) 共价有机框架材料 Au NPs/COF-TpPa-1复合纳米材料 免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] R318.08[自动化与计算机技术—控制科学与工程]