基于数字PCR的质粒核酸标准物质合作定值研究  

Resrach on the Collaborative Value Assignment of Plasmid Nucleic Acid Refernce Materials Based on Digital PCR

作　　者：牛春艳[1] 张永卓 杨佳怡 董莲华[1] 傅博强[1] 王晶[1] NIU Chun-yan;ZHANG Yong-zhuo;YANG Jia-yi;DONG Lian-hua;FU Bo-qiang;WANG Jing(National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院,北京100029

出　　处：《计量学报》2021年第11期1522-1527,共6页Acta Metrologica Sinica

基　　金：国家重点研发计划(2016YFD0500701)。

摘　　要：为研制猪繁殖与呼吸综合症病毒(PRRSV)质粒核酸标准物质,建立数字PCR方法,并联合多家实验室利用数字PCR方法对标准物质进行合作定值,探讨了合作定值过程中影响质粒定值准确性的因素,并评定了标准物质不确定度。研制的两种质粒核酸标准物质已获得国家二级标准物质证书(编号GBW(E) 091038及GBW(E)091039),可为检测方法提供定量标准,用于方法评价、产品质量控制等诸多方面。In order to develop plasmid nucleic acid reference material of porcine reproductive and respiratory syndrome virus( PRRSV),a digital PCR method was established,and several laboratories were combined to conduct cooperative evaluation of the reference material by using the digital PCR method.The factors affecting the accuracy of plasmid evaluation in the process of cooperative evaluation were discussed,and the uncertainty of the reference material was evaluated.The two reference materials have been certified with codes GBW( E) 091038 and GBW( E) 091039,which can provide quantitative standards for detection methods and can be used in method evaluation,product quality control and many other aspects.

关 键 词：计量学 猪繁殖与呼吸综合症病毒 质粒 核酸 数字PCR 标准物质 合作定值 

分 类 号：TB99[一般工业技术—计量学]