基于转录组研究光质对转色期红地球葡萄果实着色及品质的影响  

Effects of light quality on the berry coloration and quality of Red Globe grape during veraison based on transcriptome sequencing

作　　者：刘帅 张亚红[1] 徐伟荣[1] 刘鑫 袁苗 胡泽军 LIU Shuai;ZHANG Yahong;XUWeirong;LIU Xin;YUAN Miao;HU Zejun(College of Agronomy,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《果树学报》2021年第12期2045-2058,共14页Journal of Fruit Science

基　　金：国家自然科学基金(31360493)。

摘　　要：【目的】探讨不同光质对设施红地球葡萄果实着色及品质的影响,并从中挖掘出关键的光调控基因。【方法】以8年生红地球葡萄为试验材料,采用4种不同光质(红蓝光2:1、蓝光、红光、白光)补光处理设施红地球葡萄,以不补光为对照,对果实中花青素、还原糖、可滴定酸和可溶性固形物含量进行测定,并对果皮进行转录组测序。【结果】不同光质处理下果实中花青素、还原糖和可溶性固形物含量均高于对照,其中在蓝光处理下最高。利用FPKM计算基因表达量,以差异表达倍数|log2fold changes|≥1,P-adjust<0.05为筛选条件,共获得1313个差异表达基因,包括上调基因728个,下调基因585个,其中红蓝光、蓝光、红光、白光处理与对照之间的差异基因数目分别为638、434、375和320个。GO分析发现差异基因与代谢过程、细胞过程、膜、催化活性、结合等条目密切相关。KEGG分析发现不同光质通过调控葡萄光合作用、类黄酮生物合成、淀粉和蔗糖代谢等相关基因表达进而影响葡萄果实着色及品质。根据富集结果选取9个差异表达基因,经qRT-PCR验证,基因表达趋势与转录组测序结果一致。【结论】不同光质补光均能改善葡萄果实的着色及品质,其中以蓝光的效果最为明显;葡萄果实中类黄酮基因CHS、F3H及淀粉和蔗糖代谢基因INV、SPS、HK和BG的差异表达,是导致各处理葡萄果实着色及品质差异的重要原因。【Objective】Greenhouse grape has the characteristics of early berry bearing and high benefit,which has developed rapidly in Ningxia and significantly improved the economic benefit of local grape industry.Red Globe grape is the main variety of table grape in Ningxia,which has the characteristics of big ear,brilliant color,sweet taste,storage tolerance and high yield,etc.However,under the condition of protected cultivation,weak light and poor light quality will affect the color set and maturity of grapes,resulting in the decline of grape yield and quality,which restricts the sustainable development of protected grape industry.As the main environmental factor for the growth and development of protected horticultural crops,light not only affects the photosynthetic capacity of protected horticultural crops,but also participates in the regulation of various physiological processes.As an effective measure to improve lighting conditions in facilities,LED light supplement can accurately regulate spectral composition,and is widely used in facility cultivation research.Transcriptome sequencing has been applied in the study on gene structure,function and expression,it is also an important method for phenotypic correlation research,which plays an important role in exploring the molecular mechanism of plant,parsing facility grape berry coloring and quality influencing factors,and helping to improve the grape commodity value,so as to improve the economic benefits of grape planting.【Methods】The experiment was carried out in the shade of the solar greenhouse from April to September,2019.The eight-year-old Red Globe grapes were used as experimental materials,and four different kinds of light quality treatments were set up in this experiment.They were red blue light(red light:blue light=2:1),blue light,red light and white light,with no supplementary light as the control.Twelve vines with the same growth vigor were selected for each treatment and divided into 3 replicates with 4 vines in each replicate.Supplementary light lam

关 键 词：葡萄 光质 果实着色 果实品质 转录组测序 

分 类 号：S663.1[农业科学—果树学]