不同抗番茄黄化曲叶病毒基因对番茄褪绿病毒的耐性研究  

Studies on Tolerance of Different Resistant Tomato yellow leaf curl virus Genes to Tomato chlorosis virus

作　　者：范丽娜 高文征 李晓东 黄泽军[1] 国艳梅[1] 舒金帅[1] 刘磊[1] 李君明[1] 杜永臣[1] 王孝宣[1] FAN Lina;GAO Wenzheng;LI Xiaodong;HUANG Zejun;GUO Yanmei;SHU Jinshuai;LIU Lei;LI Junming;DU Yongchen;WANG Xiaoxuan(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Shaanxi Jinpeng Seed Co.,Ltd.,Yangling 712100,Shaanxi,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]陕西金鹏种业有限公司,陕西杨凌712100

出　　处：《中国蔬菜》2021年第12期26-31,共6页China Vegetables

基　　金：国家大宗蔬菜产业技术体系项目(CARS-23-A10)。

摘　　要：番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)和番茄褪绿病毒(Tomato chlorosis virus,ToCV)是近几年危害番茄较严重的病毒。本试验利用11份携带不同抗TYLCV基因的番茄材料,在TYLCV和ToCV共同侵染下对ToCV的耐性进行了研究。结果表明:携带不同抗TYLCV基因的番茄材料对ToCV耐性存在差异,只含有单个TY-2/ty-2抗性基因的材料对ToCV有一定的耐性,但株高和单株坐果数受到较大影响;而同时含有TY-1/ty-1、TY-2/ty-2、TY-3/ty-3抗性基因的材料对ToCV的耐性较强,病情指数低于43.5,且植株生长势、坐果率、果实成熟度受影响较小。对ToCV的CP基因和TYLCV的DNA-A全基因进行克隆和序列分析,结果表明复合侵染时ToCV的CP基因核苷酸序列与国内分离物同源性均达到99%以上,TYLCV的DNA-A基因核苷酸序列与国内分离物同源性均在98%以上,表明复合侵染时TYLCV和ToCV没有产生新的病毒菌株。Tomato yellow leaf curl virus(TYLCV)and Tomato chlorosis virus(ToCV)are the 2 viruses,harming tomato more serious in recent years.This experiment made use of 11 tomato materials carrying different genes resistant to TYLCV;and carried out studies on ToCV tolerance under co-infection of TYLCV and ToCV.The results showed that there were differences in tolerance to ToCV by tomato materials carrying different TYLCV resistance genes.Only materials containing single TY-2/ty-2 resistance gene showed certain tolerance to ToCV,but their plant height and number of fruit set per plant were greatly affected.However,tomato materials containing TY-1/ty-1,TY-2/ty-2,TY-3/ty-3 resistance genes at the same time had better resistance to ToCV.Their disease indexes were below 43.5,and their plant growth potential,fruit setting rate and fruit maturity were less affected.The CP gene of ToCV and DNA-A whole gene of TYLCV were cloned and analyzed,respectively.The results showed that the CP gene nucleotide sequence of ToCV at the time of compound infection was over 99%homogeneous with domestic isolates,and DNA-A gene nucleotide sequence of TYLCV was over 98%homogeneous with domestic isolates,indicating that no new viral strains occurred in TYLCV and ToCV at the time of compound infection.

关 键 词：番茄 番茄褪绿病毒 番茄黄化曲叶病毒 复合侵染 

分 类 号：S436.412.11[农业科学—农业昆虫与害虫防治]