超活化血小板裂解液对成骨细胞增殖的影响及凋亡修复作用  

Influence of super-activated platelet lysate on proliferation of osteoblasts and repairing effect on apoptosis

作　　者：崔同 吴奇剑 刘春香 廉洪宇 赵雪 李子涛 张怡 CUI Tong;WU Qi-jian;LIU Chun-xiang;LIAN Hong-yu;ZHAO-Xue;LI Zi-tao;ZHANG Yi(Hongqi Hospital Affiliated to Mudanjiang Medical University,Mudanjicing 157000,Heilongjiang Province China;不详)

机构地区：[1]牡丹江医学院附属红旗医院,黑龙江牡丹江157000 [2]杭州萧山傅氏骨伤科医院,浙江杭州311254 [3]天晴干细胞股份有限公司,黑龙江哈尔滨150028

出　　处：《中国生物制品学杂志》2021年第11期1325-1329,共5页Chinese Journal of Biologicals

基　　金：哈尔滨市松北区工业信息科技局资金项目(HSGK201913)。

摘　　要：目的探讨超活化血小板裂解液(super-activated platelet lysate,sPL)对成骨细胞增殖的影响及凋亡修复作用,以明确其能否有效应用于骨组织愈合。方法在成骨细胞hFOB.1.19中分别加入不同浓度的sPL(0%、5%、10%、20%),连续7 d计数,并绘制生长曲线,分析不同浓度sPL对成骨细胞的增殖作用;用转化生长因子-β(transforming growth factor-β,TGF-β)诱导建立成骨细胞凋亡模型,流式细胞术检测不同浓度sPL(0%、5%、10%、20%)作用36 h后,对凋亡细胞的修复作用。结果5%和10%sPL对成骨细胞具有较高的促增殖作用,5%sPL的促细胞增殖效果高于10%;20%sPL未见显著促细胞增殖作用。与0%sPL组比较,5%和10%sPL对凋亡的成骨细胞具有显著修复作用(P均<0.01);而20%sPL未见显著凋亡修复作用(P>0.05)。结论sPL可促进成骨细胞增殖以及凋亡修复,进而可达到促进骨组织愈合的效果,sPL浓度为5%时,效果更佳。Objective To investigate the influence of super-activated platelet lysate(sPL)on proliferation of osteoblasts and repairing effect on apoptosis so as to determine whether it can be effectively used for bone healing.Methods Osteoblasts hFOB.1.19 were added with sPL at various concentrations(0%,5%,10%and 20%)and counted for 7 consecutive days,based on which a growth curve was plotted to analyze the effect of different concentrations of sPL on the proliferation of osteoblasts.An osteoblast apoptosis model was established by induction with transforming growth factor-β(TGF-β).The model osteoblasts were treated with sPL at various concentrations(0%,5%,10%and 20%)for 36 h respectively,and determined for apoptosis rate by flow cytometry.Results The sPL at concentrations of 5%and 10%showed strong promoting effect on the proliferation of osteoblasts,while the effect of 5%sPL was superior to that of10%sPL.However,20%sPL showed no significantly promoting effect on the cell proliferation.The 5%and 10%sPL showed significantly repairing effect on the apoptotic osteoblasts as compared with 0%sPL(each P<0.01),while 20%sPL showed no significantly repairing effect(P>0.05).Conclusion The sPL can promote the proliferation of osteoblasts and repair apoptosis so as to promote the healing of bone tissue,of which the effect is satisfactory at a concentration of 5%.

关 键 词：超活化血小板裂解液 成骨细胞 细胞增殖 凋亡修复 

分 类 号：R458.8[医药卫生—治疗学]