Phylotranscriptomics reveals extensive gene duplication in the subtribe Gentianinae(Gentianaceae)  

作　　者：Chun-Lin Chen Lei Zhang Jia-Liang Li Xing-Xing Mao Lu-Shui Zhang Quan-Jun Hu Jian-Quan Liu Zhen-Xiang Xi 

机构地区：[1]Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education and State Key Laboratory of Hydraulics and Mountain River Engineering,College of Life Sciences,Sichuan University,Chengdu 610065,China [2]State Key Laboratory of Grassland Agro-Ecosystems,College of Life Sciences,Lanzhou University,Lanzhou 730000,China

出　　处：《Journal of Systematics and Evolution》2021年第6期1198-1208,共11页植物分类学报（英文版）

基　　金：supported by grants from the National Key Research and Development program(2017YFC0505203);National Natural Science Foundation of China(31590821,31600172,and 31700323);Fundamental Research Funds for the Central Universities(2018CDDY-S02-SCU,SCU2019D013).

摘　　要：Gene duplication plays an important role in plants for diversification and adaptation to new habitats.In this study,we aim to reconstruct the genome-scale phylogeny and identify large-scale gene duplication events for the subtribe Gentianinae(Gentianaceae),which is a great symbol of the alpine plants in the Qinghai-Tibet Plateau.We sequenced and assembled 70 transcriptomes from 67 species,representing all six recognized genera in the subtribe Gentianinae plus the closely related outgroups.Using phylogenomic approaches,the backbone relationships of Gentianinae were almost fully resolved with high bootstrap support.Although instances of conflicts were observed between nuclear and plastid phylogenies,six major clades of Gentianinae were consistently recovered in both phylogenies.In addition,we revealed a high occurrence of duplicated genes in our transcriptome assemblies.Using several gene tree reconciliation methods,we collectively identified 10 nodes in the species tree with large concentrations of duplicated genes.Further analysis indicated that many of these duplicated genes likely arose from hybrid polyploidy,which might also account for some of the topological incongruences between nuclear and plastid phylogenies in Gentianinae.

关 键 词：gene duplication Gentianinae HYBRIDIZATION PHYLOGENY TRANSCRIPTOME 

分 类 号：Q94[生物学—植物学]