慢性乙型肝炎患者高敏HBV-DNA定量与HBeAg、HBeAb水平相关性研究  

A Study on the Correlation Between High-Sensitivity HBV-DNA Quantification and HBeAg and HBeAb Levels in Patients Who Suffered Chronic Hepatitis B

作　　者：方茜 江兆涛 郭杰[1] 郭晶晶[1] 李韦杰 曲沛[1] 王雅杰 FANG Qian;JIANG Zhaotao;GUO Jie;GUO Jingjing;LI Weijie;QU Pei;WANG Yajie(Department of Clinical Laboratory,Beijing Ditan Hospital,Capital Medical University,Beijing 100015, China;Outpatient Department, Unit 96902 of PLA, Beijing 100000, China)

机构地区：[1]首都医科大学附属北京地坛医院检验科,北京100015 [2]96902部队门诊部,北京100000

出　　处：《标记免疫分析与临床》2021年第11期1824-1827,共4页Labeled Immunoassays and Clinical Medicine

基　　金：吴阶平医学基金会临床科研专项资助基金(乙肝五项定量测定临床价值探讨)(编号:320.6750.19089-75)。

摘　　要：目的分析慢性乙型肝炎患者HBV-DNA定量与HBeAg、HBeAb水平的相关性。方法选取本院2020年7月至2021年7月收治的慢性乙型肝炎患者4439例[其中HBeAg、HBV-DNA双(+)组1116例;HBeAb、HBV-DNA双(+)组1098例;HBeAg(+)、HBV-DNA(-)组1138例;HBeAg(-)、HBeAb(+)、HBV-DNA(+)组1087例]为研究对象,检测其外周血HBV-DNA、HBeAg、HBeAb水平并进行统计学分析。结果HBV-DNA与HBeAg水平呈正相关(r=0.569,P<0.001);依据HBV-DNA水平分为低表达组、中表达组、高表达组,3组HBeAg的S/CO值分别为222.84±35.70、907.33±59.81、1087.76±52.47,组间比较差异均有统计学意义;当HBeAg的cut-off值为49.13时,其ROC曲线下面积最大(AUC=0.780,P<0.01),其灵敏度和特异性分别是65.59%和80.14%;HBeAg阴性而HBV-DNA≥10^(5)的患者占研究对象的7.5%。结论当HBeAg水平升高时,外周血中HBV-DNA水平将会升高,在实际工作中,HBeAg可辅助判断病毒复制状况。特别关注HBeAg阴性高病毒血症患者,这可能是预后不良的表现。Objective To analyze the correlation between HBV-DNA quantification and HBeAg,HBeAb levels in patients who suffered chronic hepatitis B.Methods A total of 4,439 cases who admitted to our hospital from July,2020 to July,2021 were selected for the study[in which 1116 cases as the HBeAg and HBV-DNA double positive group,1098 cases as the HBeAb and HBV-DNA double positive group,1138 cases as HBeAg(+)and HBV-DNA(-)groups,and 1087 cases as HBeAg(-),HBeAb(+)and HBV-DNA(+)groups].Data was processed through statistical methods.Results HBV-DNA and HBeAg levels were positively correlated(r=0.569,P<0.001);According to HBV-DNA levels,patients were divided into the low expression group,medium expression group,and high expression group.S/CO values of the three groups of HBeAg were 222.84±35.70,907.33±59.81,and 1087.76±52.47,respectively,and the differences among groups were statistically significant;When the cut-off value of HBeAg was 49.13,the area under the ROC curve was the largest(AUC=0.780,P<0.01).The sensitivity and specificity were 65.59%and 80.14%,respectively;Patients with HBeAg negative but the value of HBV DNA greater than the fifth power was up to 7.5%in all objects.Conclusion With the level of HBeAg becoming higher,the level of HBV-DNA in peripheral blood will increase correspondingly.In clinical practice,HBeAg can help to judge the status of virus replication.Special attention on patients suffered HBeAg-negative hyperviremia should be made,which may be a sign of a poor prognosis.

关 键 词：乙型肝炎病毒DNA 抗原 抗体 

分 类 号：R512.62[医药卫生—内科学]