脓毒症晚期小鼠髓系造血情况的研究  

A Study on Myelopoiesis in Mice of Sepsis During the Late Stage

作　　者：栗雅杰[1] 王大刚 何艳群[1] 王爽 徐飞[1] 周淳[1] 王雅杰 LI Yajie;WANG Dagang;HE Yanqun;WANG Shuang;XU Fei;ZHOU Chun;WANG Yajie(Department of Clicinal Laboratory,Beijing Ditan Hosipital,Capital Medical University,Beijing 100015, China)

机构地区：[1]首都医科大学附属北京地坛医院检验科,北京100015

出　　处：《标记免疫分析与临床》2021年第11期1930-1933,共4页Labeled Immunoassays and Clinical Medicine

基　　金：吴阶平医学基金会临床科研专项资助基金(HIV-1 DNA检测临床应用价值探讨)(编号:320.6750.2020-08-35)。

摘　　要：目的利用小鼠盲肠结扎穿刺模型探讨脓毒症晚期髓系造血情况。方法将C57 BL/6小鼠分成假手术组和脓毒症组,采用盲肠结扎穿刺术(CLP)建立小鼠脓毒症模型。利用流式细胞仪检测CLP术后12d脓毒症小鼠骨髓和脾脏造血前体细胞比例的变化情况。结果与假手术组相比,CLP术后12d小鼠骨髓中的GMPs(粒-单核细胞祖细胞)、MPPs(多潜能祖细胞)、LSKs(造血干细胞Lin^(-)Sca-1^(+)c-Kit^(+))的比例显著增加;脾脏中GMPs、LSKs的比例也明显增加。结论脓毒症晚期小鼠髓系造血显著增加。Objective To investigate effects of sepsis during the late stage on myelopoiesis with mouse model which received cecal ligation and puncture(CLP).Methods The studied mice were divided into two groups.The sepsis model was developed by CLP,while Sham operation was conducted in the control group.Changes of hematopoietic stem cells and precursor cells in bone marrow and spleen were analyzed with flow cytometer 12 days after CLP.Results Compared with control group,the proportion of GMPs(granulocyte/monocyte progenitors),MPPs(multipotent progenitors),and LSKs(hematopoietic stem cells,Lin^(-)Sca-1^(+)c-Kit^(+))in bone marrow of the sepsis group increased.Meanwhile,sepsis mice also showed an increased frequency of GMPs and LSK in spleen.Conclusion Myelopoiesis of sepsis mice druing the late stage increases dramatically in both bone marrow and spleen.

关 键 词：脓毒症晚期 髓系造血 造血干祖细胞 髓外造血 

分 类 号：R631.1[医药卫生—外科学]