番茄灰叶斑病人工接种技术研究及抗病种质资源筛选  

Artificial Inoculation of Grey Leaf Spot on Tomato and Screening of Resistant Sources of Tomato

作　　者：张子君[1] 孙亚男 王涛[1] 张逸鸣[1] 邹庆道[1] ZHANG Zi-jun(Institute of Vegetable Research,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院蔬菜研究所,辽宁沈阳110161

出　　处：《园艺与种苗》2021年第11期8-10,共3页Horticulture & Seed

基　　金：辽宁省兴辽英才计划项目(XLYC1905009);辽宁省农业科学院学科建设计划(2019DD041106)。

摘　　要：[目的]探索适宜番茄灰叶斑病人工接种技术体系,并对抗病种质资源进行筛选。[方法]以番茄材料LN89为抗病材料,番茄材料LN163为感病材料,对不同接种方法、接种苗龄、接种孢子浓度、保湿时间进行系统研究。[结果]喷雾接种法是人工接种最适宜的方法,6~8叶期为植株适宜的接种时期,孢子浓度和保湿时间分别为5×10^(4)个/m L,48 h或7×10^(4)个/m L,24 h,并在抗病性鉴定中筛选出3个抗病主栽品种和5个中抗品种。[结论]该研究可为番茄灰叶斑病抗病育种工作的深入开展提供理论依据。[Objective] The aim was to construct a artificial inoculation system of grey leaf spot on tomato,and screen resistant sources of tomato. [Method] Tomato material LN89 was used as the disease-resistant material,and tomato material LN163 was used as the sensitive material. A systematic study about inoculation method,seedling age,inoculation concentration and moisture preserving time was carried out. [Result] The results showed that spraying inoculation was the proper inoculation method. The seedling age of 6 to 8 leaves was the best inoculation period. The suitable inoculation concentration and the moisture preserving time was 5×10^(4)conidia/mL,48 h or 7×10^(4)conidia/mL,24 h. Spraying inoculation method was used to identify tomato resistant sources. The results indicated that three sources were resistant,five were intermediate resistant and the others were susceptible. [Conclusion] This study provided a theoretical basis for the further development of grey leaf spot on tomato resistance breeding.

关 键 词：番茄 灰叶斑病 人工接种 抗病性鉴定 

分 类 号：S641.2[农业科学—蔬菜学]