Aroclor1254对雄性大鼠的生殖毒性效应  

Reproductive toxicity of Aroclor1254 in male rats

作　　者：陈潞萍 邢晖 冮岩[1] 赵建文[1] 邹鹏[1] 贾晓宇 Chen Luping;Xing Hui;Gang Yan;Zhao Jianwen;Zou Peng;Jia Xiaoyu(Department of Biochemistry and Molecular Biology,Shenyang Medical College,Shenyang 110034,Liaoning,China)

机构地区：[1]沈阳医学院基础部生物化学与分子生物学教研室,辽宁沈阳110034

出　　处：《实用检验医师杂志》2021年第3期152-154,共3页Chinese Journal of Clinical Pathologist

摘　　要：目的探讨Aroclor1254对成年雄性大鼠的生殖毒性效应。方法将90 d的成年雄性SD大鼠(体质量250~300 g)随机分为溶剂对照组和Aroclor1254低、中、高剂量(1、3、5 mg/kg)组,每组8只。采用腹腔注射法连续给药,制备SD大鼠生精障碍模型,溶剂对照组给予等体积玉米油,给药30 d后麻醉处死大鼠,记录各组大鼠体质量、睾丸和附睾质量,计算睾丸和附睾器官系数并检测各组大鼠给药后精子数。采用苏木素-伊红(HE)染色观察睾丸组织病理学变化。结果与溶剂对照组比较,Aroclor1254低、中、高剂量组大鼠的体质量、睾丸和附睾质量、睾丸和附睾器官系数、精子计数均随剂量升高而降低,以高剂量组降低最多〔体质量(g):278.32±5.60比301.05±4.35,睾丸质量(g):1.39±0.08比1.66±0.04,附睾质量(g):0.35±0.07比0.54±0.05,睾丸器官系数:(5.00±0.25)%比(5.51±0.10)%,附睾器官系数:(1.20±0.24)%比(1.80±0.22)%,精子计数(×10^(6)个):11.50±1.06比19.50±2.92,均P<0.05〕。Aroclor1254处理组大鼠生精小管结构破坏、排列紊乱、两生精小管间质细胞减少,且随剂量升高症状加重。结论Aroclor1254暴露可能通过破坏血睾屏障的完整性损伤大鼠生殖功能。Objective To investigate the reproductive toxicity of Aroclor1254 in adult male rats.Methods The 90-day adult male SD rats(weighing 250-300 g)were randomly divided into solvent control group,Aroclor1254 low-,mid-and high-dose(1,3,5 mg/kg)groups,with 8 rats in each group.SD rat model with spermatogenic disorder was prepared by intraperitoneal injection.The solvent control group was given corn oil.The rats were anesthetized and sacrificed 30 days after administration.The body mass,testicular and epididymal mass of rats in each group were recorded,the testicular and epididymal organ indexes were calculated,and the sperm count of rats in each group was detected.Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of testis.Results Compared with the solvent control group,the body mass,testicular and epididymal mass,testicular and epididymal organ indexes and rat sperm count of Aroclor1254 low-dose,mid-dose and high-dose groups decreased with the increase of dose,and the decrease was the most significant in high-dose group[body mass(g):278.32±5.60 vs.301.05±4.35,testicular mass(g):1.39±0.08 vs.1.66±0.04,epididymal mass(g):0.35±0.07 vs.0.54±0.05,testicular organ index:(5.00±0.25)%vs.(5.51±0.10)%,epididymal organ index:(1.20±0.24)%vs.(1.80±0.22)%,sperm count of rats(×10^(6)):11.50±1.06 vs.19.50±2.92,all P<0.05].Conclusion Exposure to Aroclor1254 may damage rat reproductive function by destroying the integrity of the blood testis barrier.

关 键 词：Aroclor1254 血睾屏障 生精障碍 生殖毒性 

分 类 号：R114[医药卫生—卫生毒理学]