荷斯坦泌乳牛粪便中益生菌的分离与鉴定  

Probiotics Isolation and Identification from Feces of Holstein Lactating Cow

作　　者：郭晓农[1,2,3] 赵诗佳 马梦慈 许朝阳 GUO Xiao-nong;ZHAO Shi-jia;MA Meng-ci;XU Zhao-yang(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Key Laboratory of Biotechnology&Bioengineering of State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China;China-Malaysia National Joint Laboratory,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,甘肃兰州730030 [3]西北民族大学生物医学研究中心中国-马来西亚国家联合实验室,甘肃兰州730030

出　　处：《西北民族大学学报（自然科学版）》2021年第4期15-20,共6页Journal of Northwest Minzu University(Natural Science)

基　　金：国家自然科学基金项目(31760242);西北民族大学2019年度中央高校基本科研业务费创新团队培育项目(31920190021);西北民族大学2020年本科生科研创新项目(XBMU-BYL20061);西北民族大学“双一流”引导专项生物工程特色学科项目(1001070204)。

摘　　要：为分离纯化与鉴定动物肠道内益生菌并进一步了解动物机体内微生态环境和微生物组成结构,采集兰州市榆中县夏官营镇的荷斯坦泌乳牛粪便,从中分离、纯化优势菌种,并通过形态观察、革兰氏染色和一系列生理生化实验进行检测.通过检测鉴定出生长性能好、产酸能力强且适合动物机体生长代谢的五株益生菌(嗜热链球菌、乳杆菌、芽孢杆菌、嗜酸乳杆菌、片球菌)和大肠杆菌.此研究以期为动物胃肠道内微生态系统及益生菌的分离鉴定提供参考.The present study aimed to understand the micro-ecological environment and microbial composition within the animal body, besides to explore the isolation, purification and identification technology of probiotics in animal intestine.The dominant strains were isolated from the feces of Holstein lactating cows collected from Xiaguanying town, Yuzhong County, Gansu province.Isolation and purification, morphological observation, gram staining and a series of physiological and biochemical experiments were carried out in the experiments.Five probiotics(Streptococcus thermophilus, Lactobacillus, Bacillus, Lactobacillus acidophilus, Phaeococcus) and Escherichia coli with good growth performance and strong acid production ability were identified.These studies may provide a basic reference for studying the microecology of animal organisms and further probiotics research.

关 键 词：益生菌 分离纯化 鉴定 理化性质检测 

分 类 号：Q939.93[生物学—微生物学] S852.6[农业科学—基础兽医学]