端粒酶活性检测研究进展  

A Review on Telomerase Activity Detection Strategies

作　　者：郭玥 吴霞[1] 夏帆 娄筱叮 GUO Yue;WU Xia;XIA Fan;LOU Xiao-ding(State Key Laboratory of Biogeology and Environmental Geology,Engineering Research Center of Nano-Geomaterials of Ministry of Education,Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430078,China)

机构地区：[1]中国地质大学(武汉)生物地质与环境地质国家重点实验室,材料与化学学院,纳米矿物材料及应用教育部工程研究中心,湖北武汉430078

出　　处：《分析测试学报》2021年第12期1819-1826,共8页Journal of Instrumental Analysis

基　　金：国家重点研发计划纳米科技专项(2020YFA0211200);国家自然科学基金面上项目(22090050,21974128,21874121,52003257,21703210);湖北省自然科学基金杰出青年基金(2019CFA043,2020CFA037)。

摘　　要：端粒酶活性检测方法经过近30年的不断改革与发展,取得了巨大突破。该文重点综述了近3年的端粒酶活性检测方法,其中包括化学反应发光法、电化学法、荧光分析法、比色法等,旨在更加全面地了解目前端粒酶活性检测的研究进展,为设计新的端粒酶活性检测方法和克服端粒酶活性检测方法在临床试验中的挑战提供思路。Telomerase is a ribonucleoprotein which can catalyze the addition of single strand repeat units(TTAGGG)n onto the 3'ends of telomeres in the eukaryotic chromosomes,preserving telomeric length and stability.Compared to human somatic cells,it is testified that telomerase activity is overexpressed in over 85%of all known human cancer cells,indicating that telomerase would be used as a biomarker for the detection of tumor cells.Therefore,a reliable method for telomerase detecting plays a crucial role in the diagnosis and treatment of malignant tumors.Since telomerase was discovered in 1985,telomerase activity detection methods have made great breakthroughs in the following 30 years of continuous reform and development.In this paper,the methods for detecting telomerase activity in the past three years were reviewed,such as chemiluminescence method,electrochemistry method,fluorescence analysis method as well as colorimetry.To cut the long story short,the aim of this paper is to have a more comprehensive understanding of the current research progress of telomerase activity detection,which may be of great help to the design of new telomerase activity detection protocols and provide some brand new ideas for overcoming the challenges of telomerase activity detection methods in clinical trials in the future.

关 键 词：端粒酶活性 化学反应发光法 电化学法 荧光分析法 比色法 

分 类 号：O657.3[理学—分析化学] O629.7[理学—化学]