脂肪干细胞移植对老年小鼠脂肪组织免疫微环境的影响  

Effect of adipose-derived stem cell transplantation on the immune microenvironment of adipose tissue in aged mice

作　　者：吴思怡 吴琼[1] Wu Siyi;Wu Qiong(College of Life Sciences,Guangxi Normal University,Guilin 541004,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西师范大学生命科学学院,广西壮族自治区桂林市541004

出　　处：《中国组织工程研究》2022年第24期3773-3778,共6页Chinese Journal of Tissue Engineering Research

基　　金：广西自然科学基金项目(2020GXNSFAA259049),项目负责人:吴琼;广西研究生教育创新计划项目(YCSW2021115),项目参与人:吴思怡。

摘　　要：背景:随着脂肪干细胞的广泛应用,探寻其应用调控机制,有利于拓宽材料的选择。目的:探讨脂肪干细胞移植对老年小鼠脂肪组织免疫微环境的影响及其在衰老相关性肥胖中的作用机制。方法:分离纯化得到小鼠脂肪干细胞,通过腹腔注射移植到18月龄老年小鼠体内,腹腔注射等量PBS的18月龄老年小鼠作为老年对照组,腹腔注射等量PBS的2月龄小鼠作为青年对照组。移植4周后,记录各组小鼠食物摄取量、体质量变化,流式细胞术分析脂肪、脾、骨髓、外周血中免疫细胞丰度变化,qRT-PCR、ELISA检测脂肪组织和血清中白细胞介素6、肿瘤坏死因子α水平。结果与结论:①与老年对照组比较,脂肪干细胞移植组老年小鼠体质量降低了(1.94±0.26)g;②流式细胞术丰度分析结果显示,脂肪干细胞移植组老年小鼠中髓源抑制细胞丰度减少,T细胞丰度增加,B细胞和NK细胞丰度趋向青年对照组发展;③qRT-PCR和ELISA实验结果显示,脂肪干细胞移植组老年小鼠脂肪组织和血清中白细胞介素6、肿瘤坏死因子α表达水平显著下调;④结果表明,脂肪干细胞移植能够通过对老年小鼠免疫微环境的调控来抑制炎症衰老小鼠肥胖的发生。BACKGROUND:With the wide application of adipose-derived stem cells,exploring the regulatory mechanism of adipose stem cells is helpful to broaden the choice of materials.OBJECTIVE:To investigate the effect of adipose-derived stem cell transplantation on the immune microenvironment of adipose tissue in aged mice and its mechanism of action in aging-associated obesity.METHODS:Mouse adipose-derived stem cells were isolated and purified,and transplanted into aged mice at the age of 18 months by intraperitoneal injection.The 18-month-old mice with the same amount of PBS intraperitoneally were used as the old control group,and the 2-month-old mice with the same amount of PBS intraperitoneally were used as the young control group.At 4 weeks after transplantation,changes in food intake and body weight were recorded in each group,and changes in immune cell abundance in adipose,spleen,bone marrow and peripheral blood were analyzed by flow cytometry.The levels of interleukin-6 and tumor necrosis factor-αin adipose tissue and serum were detected using qRT-PCR and ELISA.RESULTS AND CONCLUSION:(1)The body weight of adipose-derived stem cells-transplanted aged mice decreased by(1.94±0.26)g compared with aged controls.(2)The results of flow cytometry abundance analysis showed that the abundance of myeloid-derived suppressor cells decreased,the abundance of T cells increased,and the abundance of B cells and NK cells tended to develop in adipose-derived stem cells-transplanted aged mice toward young controls.(3)The results of qRT-PCR and ELISA experiments showed that expression levels of interleukin-6 and tumor necrosis factor-αwere significantly down-regulated in the adipose tissue and serum of adipose-derived stem cells-transplanted aged mice.(4)In summary,adipose-derived stem cell transplantation was able to suppress the development of obesity in inflammatory aging mice through the modulation of the immune microenvironment in aged mice.

关 键 词：脂肪干细胞 移植 脂肪组织 免疫 微环境 炎症 衰老 肥胖 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]