哇巴因体外诱导人骨髓间充质干细胞的成骨分化  

Ouabain induces in vitro differentiation of human bone marrow mesenchymal stem cells into osteoblasts

作　　者：崔寅鹏 郭艾[2] 马立峰[2] 刘振江 Cui Yinpeng;Guo Ai;Ma Lifeng;Liu Zhenjiang(Department of Orthopedics,Children’s Hospital,Capital Institute of Pediatrics,Beijing 100020,China;Department of Orthopedics,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都儿科研究所附属儿童医院骨科,北京市100020 [2]首都医科大学附属北京友谊医院骨科,北京市100050

出　　处：《中国组织工程研究》2022年第24期3796-3801,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:哇巴因作为Na+/K+-ATPase的配体可与其结合调节细胞的增殖代谢,研究哇巴因对骨髓间充质干细胞成骨分化的影响,可为骨缺损、骨病的治疗提供思路。目的:探讨不同浓度Na+/K+-ATPase配体哇巴因对人骨髓间充质干细胞成骨分化的影响。方法:以人工髋关节置换手术中废弃的新鲜骨髓为实验材料,采用全骨髓直接贴壁培养法分离纯化获得人骨髓间充质干细胞,作为诱导成骨分化的实验细胞;成骨诱导前以不同浓度哇巴因(10,100,1000 nmol/L)处理人骨髓间充质干细胞24 h,未经药物处理的人骨髓间充质干细胞直接进行诱导作为对照组,然后成骨诱导21 d后进行茜素红染色,使用Image Pro Plus软件测量染色面积比例,比较各组产生钙化结节量的差异。结果与结论:与对照组比较,低浓度(10 nmol/L)哇巴因刺激人骨髓间充质干细胞后,茜素红染色钙化结节数量增多,染色面积高于对照组(P<0.05);高浓度(100,1000 nmol/L)哇巴因刺激后钙化结节数量减少,染色面积低于对照组(P<0.05),且浓度越高钙化结节数量越少。结果表明,人工髋关节置换术中废弃的新鲜骨髓可作为分离纯化得到人骨髓间充质干细胞的实验材料,低浓度哇巴因可促进人骨髓间充质干细胞成骨分化,高浓度哇巴因抑制人骨髓间充质干细胞成骨分化。BACKGROUND:As a ligand of Na+/K+-ATPase,ouabain can be combined with it to regulate cell proliferation and metabolism.Studying the effect of ouabain on the osteogenic differentiation of bone marrow mesenchymal stem cells can provide ideas for the treatment of bone defects and bone diseases.OBJECTIVE:To explore the effects of different concentrations of Na^(+)/K^(+)-ATPase ligand ouabain on the osteogenic differentiation of human bone marrow mesenchymal stem cells.METHODS:Human bone marrow mesenchymal stem cells were isolated from fresh bone marrow samples from patients that experienced hip replacement,using the whole bone marrow direct adherence method,as experimental cells for inducing osteogenic differentiation.Before osteoinduction,human bone marrow mesenchymal stem cells were treated in different concentrations of ouabain(10,100,and 1000 nmol/L)for 24 hours.The untreated human bone marrow mesenchymal stem cells were used as the control group to induce directly.At 21 days after osteoinduction,alizarin red staining was performed.The proportion area was measured using Image Pro Plus software to compare the differences in the amount of calcified nodules produced in each group.RESULTS AND CONCLUSION:Compared with the control group,as human bone marrow mesenchymal stem cells in vitro treated with ouabain in low concentration(10 nmol/L),the number of calcified nodules increased after alizarin red staining and the staining areas were higher than that in the control group(P<0.05).The number of calcified nodules observed after treatment in the high concentration group(100,1000 nmol/L)was reduced,and the measured stained area was smaller than that in the control group(P<0.05).The higher the concentration,the fewer the calcifiednodules were.It is concluded that the fresh bone marrow form hip arthroplasty can be used as a test material for isolating human bone marrow mesenchymal stem cells.Low concentrations of ouabain promote differentiation of human bone marrow mesenchymal stem cells into osteoblasts,while a high concen

关 键 词：干细胞 骨髓 人骨髓间充质干细胞 哇巴因 Na+/K+-ATPase 成骨分化 人工髋关节置换 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]