Molecular basis of the silkworm mutant re^(l) causing red egg color and embryonic death  

作　　者：Jiang-Wen Luo Er-Xia An Ya-Ru Lu Ling Yang Ting-Ting Gai Song-Zhen He Song-Yuan Wu Hai Hu Chun-Lin Li Cheng Lu Xiao-Ling Tong Fang-Yin Dai 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,Key Laboratory of Sericultural Biology and Genetic Breeding,College of Biotechnology,Southwest University,Chongqing,400715 China

出　　处：《Insect Science》2021年第5期1290-1299,共10页昆虫科学（英文版）

基　　金：the National Natural Sci-ence Foundation of China(No.31830094);the Hi-Tech Research and Development 863 Program of China Grant(No.2013AA 102507);funds from the China Agri-culture Research System(No.CARS-18-ZJ0102).

摘　　要：The coloration and hatchability of insect eggs can affect individual and population survival.However,few genetic loci have been documented to affect both traits,and the genes involved in regulating these two traits are unclear.The silkworm recessive mutant re^(l)shows both red egg color and embryo mortality.We studied the molecular basis of the re^(l)phenotype formation.Through genetic analysis,gene screening and sequencing,we found that two closely linked genes,BGIBMGA003497(Bmre)and BGIBMG4003697(Bm-Semala),control egg color and embryo mortality,respectively.Six base pairs of the Bm-re gene are deleted in its open reading frame,and BmSemala is expressed at abnormally low levels in mutant re^(l).BmSemala gene function verification was performed using RNA interference and clustered randomly interspersed palindromic repeats(CRISPR)/CRISPR-associate protein 9.Deficiency of the BmSemala gene can cause the death of silkworm embryos.This study revealed the molecular basis of silkworm re^(l)mutant formation and indicated that the Semala gene is essential for insect embryo development.

关 键 词：BmSemala CRISPR/Cas9 embryonic mortality red egg SILKWORM 

分 类 号：Q78[生物学—分子生物学]