Transcription factor CncC potentially regulates the expression of multiple detoxification genes that mediate indoxacarb resistance in Spodoptera litura  

作　　者：Li Shi Yao Shi Meng-Fei Liu Ya Zhang Xiao-Lan Liao 

机构地区：[1]Hunan Provincial Engineering and Technology Research Center for Bio-pesticide and Formulation Processing,College of Plant Protection,Hunan Agricultural University,Changsha,China [2]Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Changsha,China

出　　处：《Insect Science》2021年第5期1426-1438,共13页昆虫科学（英文版）

基　　金：the National Natural Science Foundation of China(31801764);the Natural Science Foundation of Hunan Province,China(2019JJ50230);the Scientific Research Fund of Hunan Provincial Education Department(18B 105);China Postdoctoral Science Foundation(2019M652770);the Youth Fund Project of Hunan Agricultural University(17QN34)。

摘　　要：The xenobiotic transcription factor cap‘n’collar isoform C(CncC)is considered the central regulator of antioxidant and detoxification genes.Previous research indicated that CncC regulates three-phase enzymes responsible for insecticide resistance.In this study,the SlituCncC gene from Spodoptera litura was obtained and characterized.Quantitative polymerase chain reaction(qPCR)analysis showed that SlituCncC was expressed in all developmental stages and tissues,but was highly expressed in 3rd-and 4th-instar larvae,and in the Malpighian tubule,fat body,and midgut.In addition,SlituCncC was up-regulated and more highly induced with indoxacarb treatment in the indoxacarb-resistant strains compared with the susceptible strain.RNA interference-mediated gene silencing of SlituCncC significantly increased mortality of S.liura when exposed to in-doxacarb.Furthermore,comparative transcriptome analysis showed that 842 genes were down-regulated and 127 genes were up-regulated in SlituC"ncC knockdown S.litura.Further analysis indicated that 18 three-phase enzymes were identified in the down-regulated genes,of which seven were associated with indoxacarb resistance in S.litura.qPCR analysis confirmed that expression of six of these seven genes was consistent with RNA se-quencing data.All six detoxification genes were induced by indoxacarb,and the expression patterns were similar to that of SlituCncC.Finally,the CneC-Maf binding site was predicted in all six gene promoters.This study indicates that the transcription factor SlituCncC may regulate multiple detoxification genes that mediate indoxacarb resistance in S.litura.

关 键 词：Spodoptera litura indoxacarb resistance transcription factor detoxification genes RNA interference RNA sequencing 

分 类 号：Q96[生物学—昆虫学]