不同麻醉药对睡眠剥夺大鼠脑脊液Aβ和tau蛋白浓度的影响  

Effects of different anesthetics on concentrations of Aβ and tau protein in cerebrospinal fluid of sleep deprived rats

作　　者：焦雅丽 解雅英[2] 于建设[2] Jiao Yali;Xie Yaying;Yu Jianshe(Department of Anesthesiology,Inner Mongolia Maternity and Child Health Care Hospital,Hohhot 010010,China;Department of Anesthesiology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010010,China)

机构地区：[1]内蒙古自治区妇幼保健院麻醉科,呼和浩特010010 [2]内蒙古医科大学附属医院麻醉科,呼和浩特010010

出　　处：《中华麻醉学杂志》2021年第10期1218-1221,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价不同麻醉药对睡眠剥夺大鼠脑脊液β淀粉样蛋白(Aβ)和tau蛋白浓度的影响。方法SPF级健康雄性SD大鼠40只,3~4月龄,体重230~280 g,采用随机数字表法分为5组(n=8):对照组(C组)、睡眠剥夺组(SD组)、丙泊酚组(P组)、右美托咪定组(D组)和氯胺酮组(K组)。采用改良的多平台睡眠剥夺模型。P组、D组和K组睡眠剥夺72 h时分别腹腔注射丙泊酚100 mg/kg、右美托咪定100 mg/kg、氯胺酮80 mg/kg维持麻醉3 h,C组进入大平台自由活动72 h。于麻醉3 h时取脑脊液,采用ELISA法测定Aβ和tau蛋白浓度。结果与C组比较,SD组、P组、K组和D组大鼠脑脊液Aβ和tau蛋白浓度升高(P<0.05);与SD组比较,P组和K组大鼠脑脊液Aβ和tau蛋白浓度升高,D组大鼠脑脊液Aβ和tau蛋白浓度降低(P<0.05);与P组和K组比较,D组大鼠脑脊液Aβ和tau蛋白浓度降低(P<0.05)。结论右美托咪定可降低睡眠剥夺大鼠脑脊液Aβ和tau蛋白浓度,而丙泊酚和氯胺酮作用相反。Objective To evaluate the effects of propofol,dexmedetomidine and ketamine on oncentrations ofβ-amyloid peptide(Aβ)and tau in cerebrospinal fluid(CSF)of sleep-deprived rats.Methods Forty SPF healthy male Sprague-Dawley rats,aged 3-4 months,weighing 230-280 g,were divided into 5 groups(n=8 each)using a random number table method:control group(group C),sleep deprivation group(SD),propofol group(group P),dexmedetomidine group(group D)and ketamine group(group K).The sleep deprivation was induced using the improved multi-platform sleep deprivation model.Propofol 100 mg/kg,dexmedetomidine 100 mg/kg and ketamine 80 mg/kg were intraperitoneally injected at 72 h of sleep deprivation to maintain anesthesia for 3 h in P,D and K groups,respectively.Group C entered the large platform for 72 h free activity.The CSF was collected at 3 h of anesthesia for measurement of concentrations of Aβand tau protein by enzyme-linked immunosorbent assay.Results The concentrations of Aβand tau protein in CSF were significantly higher in SD,P,K and D groups than in group C(P<0.05).Compared with group SD,the concentrations of Aβand tau protein in CSF were significantly increased in P and K groups,and the concentrations of Aβand tau protein in CSF were significantly decreased in group D(P<0.05).Conclusion Dexmedetomidine can decrease the the concentrations of Aβand tau protein in CSF of sleep deprived rats,while propofol and ketamine lead to the opposite effect.

关 键 词：麻醉药 睡眠剥夺 脑脊髓液 淀粉样蛋白 TAU蛋白质类 

分 类 号：R614[医药卫生—麻醉学]